Garden cress (Lepidium sativum L.), a member of the Cruciferae family, is widely planted across the world, especially in India, Europe, and the United States. It has been respected as a key medicinal plant from the time of the Vedic culture. Lepidium sativum Linn. seeds were tested for their efficacy as an antibacterial agent against pathogens found in food. The active components were extracted from the powdered dry seeds using chloroform, ethyl acetate, methanol, and dichloromethane. The antibacterial activity of various doses of the extracts was evaluated using agar well diffusion. We also estimated the MIC and MBC for the most effective extract using the tube dilution technique and the subculturing method, respectively. One of the most common mosquito species that carries Plasmodium falciparum is the Anopheles gambiae sensu lato, which may be combated by sprinkling fields with Lepidium sativum seeds. Scientists have begun to extract essential oils from Lepidium sativum and evaluate their bio-potential against juvenile and adult Anopheles gambiae as part of an attempt to identify ecologically viable vector control tactics. Based on the findings, it is clear that L. sativum essential oil effectively kills An. gambiae. Although field application on a wide scale is necessary for An. gambiae population control, more work has to be done in formulation and assessment.
Objective: The study aimed to isolation and diagnosis Clostridium perfringens from Al-Rammathia general hospital environment (lobbies and operating theaters) and determining the percentages of Clostrdium perfringens contamination in lobbies and operating theaters and comparing them with each other. Methodology: Collected in the current study (188) sample of the hospital environment, which included walls and flooring tools and beds for lobbies and operations room, in Al-Rumaitha General hospital and for a period ranging from October (2021) until April(2022(these samples were taken by sterile cotton swabs and used to transport them to the microbiology laboratory at the College of Science / University of Muthanna in media of Clostridium perfringens broth to perpetuate them, then the medium containing the sample was boiled in a water bath at 100 degrees Celsius for 20 minutes and incubated anaerobic using Anaerobic jar and gas-generating bags (Gas pak). Which provides the anaerobic conditions necessary for bacterial growth through oxygen reduction inside the container’s atmosphere at a temperature of 37 for a period ranging from 24-48 hours. It was then re-implanted on the final media for purification before being used in diagnostic testing.
The study aimed to estimate the main histological changes in the deferent organs in local domestic rabbits after infected with Pseudomonas aeruginosa after isolated from Sawa lack. The experimental animals were divided into two main groups, a group that considers the treated group that treated with Pseudomonas aeruginosa. The B group which consider as a control group. The histological results after infected in deferent organs were very prominent in all infected organs. The histological results of the lung showed acute degeneration in the tissue structures of lung, there was prominent degeneration in the wall of alveoli. The result showed disappeared the normal tissue structures of the lung. The tissue section of the liver after infected with Pseudomonas aeruginosa showed the liver parenchyma has blood congestions, the hepatic cords were randomly distributions around the central vein. The tissue section showed high aggregations of kupffer cells a rounded the abnormal hepatic cords. The cortical region of the kidney characterized some features which included the abnormal shape of the renal corpuscle, the renal tubules have irregular wide lumen filled with infiltrate and noted Red Blood Corpuscles. The tissue section showed blood congestion between the intestinal villi of the small intestine and disappeared from the basement membrane under the epithelial layer. These results were shown that the Production of extracellular proteases increases the organism's virulence by assisting in bacterial adherence and invasion.
S.agalactiae is a Gram-positive, spherical bacteria that causes postpartum sepsis, endometritis, chorioamnionitis, and premature birth in pregnant women.Streptococcus agalactiae, often known as group B streptococci (GBS), is a dangerous disease that can infect both humans and animals (GBS) is an important bacterial pathogen that causes a wide range of infections including eonatal sepsis, meningitis, pneumonia and soft tissue or urinary tract infections.The present study demonstrated that S. agalactiae isolates obtained from vaginal samples showed a high rate of resistance to tetracycline. Objectives: The study aimed to investigate streptococcus aqalctaein pregnancy woman by isolating and diagnosing them from rectovaginal colonization .Result We were collected 206 swab from pregnant female this collected was during gestation period (35-37)week and were collected from different age that including (≤25 ) years,and (≥25years .this sample collected from different hospital included (AL_Rumaitha Hospital, womans and childrens hospital ).the collected swab had transfer swabs by Amis transported medium and give to lab. After that diagnosis by microscobic and biochemical test. S. agalactiae was spread through pregnant female and infection the infant.
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