Aim: To analyze the association and susceptibility of Single Nucleotide Polymorphisms (SNPs) in the DRD2 and BDNF genes with BED in patients with weight regain in the postoperative period of bariatric surgery. Methods: 177 individuals who underwent bariatric surgery with weight regain were evaluated and divided into two groups according to the BED diagnostic. The individuals were submitted to an anthropometric evaluation, analysis of the presence of BED using a validated questionnaire, and blood collection for genotyping of the polymorphisms rs6265 (BDNF) and rs1800497 (DRD2) by Real-Time Polymerase Chain Reaction (RT-PCR). Results: the presence of wild-type alleles for rs1800497 (CC) and rs6265 (GG) were more frequent in patients without BED. Nevertheless, the presence of one or two variant alleles for rs1800497 (CT+TT) and rs6265 (GA+AA) was more frequent in patients with BED. The combination of the two studied SNPs prevailed in patients with BED.Conclusions: In the presence of polymorphisms rs1800497 in the DRD2 gene and rs6265 in the BDNF gene, isolated and/or combined could occur an additional risk for the development of BED in patients with obesity, especially in the context of weight regain.
Background/Aim: We studied the molecular pathogenesis of obesity, involving complex interactions between environmental and genetic factors, with a focus on the leptin gene. It was our aim to characterize the LEP -2548G>A leptin polymorphism and lipid profile in obese and normal-weight individuals. Methods: A total of 212 individuals were divided into the study group including 136 obese patients (body mass index, BMI ≥30) and the control group with 76 normal-weight individuals (BMI >18.5 and ≤24.9). DNA was amplified by polymerase chain reaction and restriction fragment length polymorphism. The lipid profile was analyzed by enzymatic colorimetric methods. The level of significance was set at p < 0.05. Results: There was a prevalence of the GA genotype in both groups. However, comparative group analysis showed an association of the recessive model (AA+GA) with increased triglycerides (TG) and decreased high-density lipoprotein cholesterol (HDL-C) levels in the study group. Conclusion: This study did not confirm an association between obesity and the LEP -2548G>A polymorphism. However, AA+GA genotypes, in the presence of obesity, seem to contribute to a reduction in HDL-C and an increase in TG compared with normal-weight individuals. This should be confirmed in further studies.
Background and study aims Endoscopic procedure using argon plasma coagulation (APC) promotes a progressive reduction in gastrojejunal anastomosis diameter. The present study aimed to evaluate the efficacy of the APC in patients with weight regain in the postoperative periods of gastric bypass. Patients and methods This was a randomized controlled trial conducted with 66 patients who were randomly assigned selected (using lottery method) and divided into two groups: study group (SG), 38 patients (APC treatment); and control group (CG), 28 patients (only endoscopy procedure). We considered 30 days,180 days, and one year as short-term, medium-term, and long-term, respectively. The parameters analyzed were total weight loss (TWL), excess weight loss (%EWL), total weight loss (%TWL), and reduction of weight regain (%RWR). Furthermore, a biopsy for neoplastic histological changes was carried out for the APC group. For statistical analysis, values of P < 0.05 were considered significant. Results The %TWL and %RWR were higher in the SG in short, medium, and long terms, when compared to the same periods in the CG (P < 0.001). One year after follow-up, the final weight did not reach the statistical difference between groups. Biopsy performed in SG 1 year after APC did not reveal neoplastic histological changes. Conclusions APC effectively treats weight regain after bariatric surgery in the short and medium-term. An important "new" weight gain was observed in the long-term, showing that obesity is a chronic disease that requires multidisciplinary and family care for life. Also, APC is a safe procedure with low adverse event rates.
Objectives: Verify the frequency of polymorphisms related to obesity and Binge Eating Disorder (BED) in DRD2 and BDNF genes in patients undergoing bariatric surgery with weight regain above 10% of the weight lost. Methods: Evaluation of 177 individuals undergoing bariatric surgery with weight regain, divided into two groups: Group 1: individuals with BED; Group 2: individuals without BED. The individuals were submitted to anthropometric evaluation, analysis of the presence of BED using a validated questionnaire, and blood collection for genotyping of the polymorphisms, rs6265 (BDNF) and rs1800497 (DRD2). The Kolmogorovi-Smirnov, t-test, chi-square, Mann Whitney, Pearson correlation were used for statistical analysis. Results: CC genotypes for rs1800497 polymorphism, and GG for rs6265 polymorphism were more frequent in patients without the disorder. The presence of at least one T allele (DRD2) and at least one A allele (BDNF) was more frequent in patients with BED. The combination of AA + GA + TT + TC genotypes prevailed in patients with the disorder. Conclusions: The CC (DRD2) and GG (BDNF) genotypes suggest protection for patients against BED, while genotypes that have at least one T allele (DRD2) and one A allele (BDNF) suggest greater risk and appear to act in synergism.Level of evidence: III (evidence obtained from case-control analytic study).
Aim: To analyze the association and susceptibility of Single Nucleotide Polymorphisms (SNPs) in the DRD2 and BDNF genes with BED in patients with weight regain in the postoperative period of bariatric surgery. Methods: 177 individuals who underwent bariatric surgery with weight regain were evaluated and divided into two groups according to the BED diagnostic. The individuals were submitted to an anthropometric evaluation, analysis of the presence of BED using a validated questionnaire, and blood collection for genotyping of the polymorphisms rs6265 (BDNF) and rs1800497 (DRD2) by Real-Time Polymerase Chain Reaction (RT-PCR). Results: the presence of wild-type alleles for rs1800497 (CC) and rs6265 (GG) were more frequent in patients without BED. Nevertheless, the presence of one or two variant alleles for rs1800497 (CT+TT) and rs6265 (GA+AA) was more frequent in patients with BED. The combination of the two studied SNPs prevailed in patients with BED. Conclusions: In the presence of polymorphisms rs1800497 in the DRD2 gene and rs6265 in the BDNF gene, isolated and/or combined could occur an additional risk for the development of BED in patients with obesity, especially in the context of weight regain.Level of evidence: III (evidence obtained from the case-control analytic study).
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