Aim: To analyze the association and susceptibility of Single Nucleotide Polymorphisms (SNPs) in the DRD2 and BDNF genes with BED in patients with weight regain in the postoperative period of bariatric surgery. Methods: 177 individuals who underwent bariatric surgery with weight regain were evaluated and divided into two groups according to the BED diagnostic. The individuals were submitted to an anthropometric evaluation, analysis of the presence of BED using a validated questionnaire, and blood collection for genotyping of the polymorphisms rs6265 (BDNF) and rs1800497 (DRD2) by Real-Time Polymerase Chain Reaction (RT-PCR). Results: the presence of wild-type alleles for rs1800497 (CC) and rs6265 (GG) were more frequent in patients without BED. Nevertheless, the presence of one or two variant alleles for rs1800497 (CT+TT) and rs6265 (GA+AA) was more frequent in patients with BED. The combination of the two studied SNPs prevailed in patients with BED.Conclusions: In the presence of polymorphisms rs1800497 in the DRD2 gene and rs6265 in the BDNF gene, isolated and/or combined could occur an additional risk for the development of BED in patients with obesity, especially in the context of weight regain.

Background and study aims Endoscopic procedure using argon plasma coagulation (APC) promotes a progressive reduction in gastrojejunal anastomosis diameter. The present study aimed to evaluate the efficacy of the APC in patients with weight regain in the postoperative periods of gastric bypass. Patients and methods This was a randomized controlled trial conducted with 66 patients who were randomly assigned selected (using lottery method) and divided into two groups: study group (SG), 38 patients (APC treatment); and control group (CG), 28 patients (only endoscopy procedure). We considered 30 days,180 days, and one year as short-term, medium-term, and long-term, respectively. The parameters analyzed were total weight loss (TWL), excess weight loss (%EWL), total weight loss (%TWL), and reduction of weight regain (%RWR). Furthermore, a biopsy for neoplastic histological changes was carried out for the APC group. For statistical analysis, values of P < 0.05 were considered significant. Results The %TWL and %RWR were higher in the SG in short, medium, and long terms, when compared to the same periods in the CG (P < 0.001). One year after follow-up, the final weight did not reach the statistical difference between groups. Biopsy performed in SG 1 year after APC did not reveal neoplastic histological changes. Conclusions APC effectively treats weight regain after bariatric surgery in the short and medium-term. An important "new" weight gain was observed in the long-term, showing that obesity is a chronic disease that requires multidisciplinary and family care for life. Also, APC is a safe procedure with low adverse event rates.

Objectives: Verify the frequency of polymorphisms related to obesity and Binge Eating Disorder (BED) in DRD2 and BDNF genes in patients undergoing bariatric surgery with weight regain above 10% of the weight lost. Methods: Evaluation of 177 individuals undergoing bariatric surgery with weight regain, divided into two groups: Group 1: individuals with BED; Group 2: individuals without BED. The individuals were submitted to anthropometric evaluation, analysis of the presence of BED using a validated questionnaire, and blood collection for genotyping of the polymorphisms, rs6265 (BDNF) and rs1800497 (DRD2). The Kolmogorovi-Smirnov, t-test, chi-square, Mann Whitney, Pearson correlation were used for statistical analysis. Results: CC genotypes for rs1800497 polymorphism, and GG for rs6265 polymorphism were more frequent in patients without the disorder. The presence of at least one T allele (DRD2) and at least one A allele (BDNF) was more frequent in patients with BED. The combination of AA + GA + TT + TC genotypes prevailed in patients with the disorder. Conclusions: The CC (DRD2) and GG (BDNF) genotypes suggest protection for patients against BED, while genotypes that have at least one T allele (DRD2) and one A allele (BDNF) suggest greater risk and appear to act in synergism.Level of evidence: III (evidence obtained from case-control analytic study).

IntroductionNutriepigenetic markers are predictive responses associated with changes in “surrounding” environmental conditions of humans, which may influence metabolic diseases. Although rich in calories, Western diets could be linked with the deficiency of micronutrients, resulting in the downstream of epigenetic and metabolic effects and consequently in obesity. Zinc (Zn) is an essential nutrient associated with distinct biological roles in human health. Despite the importance of Zn in metabolic processes, little is known about the relationship between Zn and epigenetic. Thus, the present study aimed to identify the epigenetic variables associated with Zn daily ingestion (ZnDI) and serum Zinc (ZnS) levels in women with and without obesity.Materials and MethodsThis is a case-control, non-randomized, single-center study conducted with 21 women allocated into two groups: control group (CG), composed of 11 women without obesity, and study group (SG), composed of 10 women with obesity. Anthropometric measurements, ZnDI, and ZnS levels were evaluated. Also, leukocyte DNA was extracted for DNA methylation analysis using 450 k Illumina BeadChips. The epigenetic clock was calculated by Horvath method. The chip analysis methylation pipeline (ChAMP) package selected the differentially methylated regions (DMRs).ResultsThe SG had lower ZnS levels than the CG. Moreover, in SG, the ZnS levels were negatively associated with the epigenetic age acceleration. The DMR analysis revealed 37 DMRs associated with ZnDI and ZnS levels. The DMR of PM20D1 gene was commonly associated with ZnDI and ZnS levels and was hypomethylated in the SG.ConclusionOur findings provide new information on Zn's modulation of DNA methylation patterns and bring new perspectives for understanding the nutriepigenetic mechanisms in obesity.

No abstract