In addition to the previously reported alkaloids from Mitragyna speciosa a number of additional oxindole alkaloids were isolated, three of which are new. These have been named mitrafoline, isomitrafkline and isospeciofoline. Mitragynine oxindoles A and B were found for the first time in plant material while corynoxeine and the corynoxines were found for the first time in the genus Mitragyna. Examination of thirteen monthly samples of leaves showed that mitragynine and paynantheine remained the dominant indole alkaloids of the mature plant throughout the entire period of collection. TRAGER, LEE, PHILLIPSON, HADDOCK DWUMA-BADU and BECKETT [4]. Since then number of other sample: of Mitragyna speciosa consisting o: leaves and young twigs from Thailanc have been examined. More sensitivc
Shellard, Houghton, Resha Experimental and Results MaterialsYoung plants 24 months old (up to 1 m high) obtained by germination of seeds obtained from Bangkok and subsequently grown in the hot houses at Chelsea Physic Garden, the temperature being maintained 24-26' C and the relative humidity about 60°/o. MethodsThe method used was that described by SHELLARD and HOUGHTON [2] except that densitometry was not used for purposes of quantitation. Instead, personal observation of the coloured alkaloidal spots on TLC using 0.2 M FeC1, in 35 O/ o HClO, enabled an approximate comparative analysis of the amounts of the alkaloids present in various parts of the plant to be made. The score 10 was
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