ABSTRACT. Abilities of the polymorphic DNA markers amplified by RAPD (Randomly Amplified Polymorphic DNA) and ISSR (Inter Simple Sequence Repeat) were studied, detected and systematized breeding lines and expanding molecular variabilities and molecular phylogenetic lines of the cultivarietal 'Marguerites' of the Argyranthemum lineage and especially the intergeneric hybrids of 'Marguerite' X Glebionis carinata Tzvelev or G. coronaria (L.) Spach. The reproducible polymorphic bands were generated by RAPD-PCR using RAPD primer OPC-04, in respective species of Glebionis and the intergeneric hybrids of the 'Marguerites.' The primer OPC-04 made it possible to identify either two intergeneric hybrids of 'Marguerite' X G. carinata or 'Marguerite' X G. coronaria. The polymorphic bands were amplified by ISSR-PCR in the breeding lines and the cultivars of the 'Marguerite,' while they were not amplified by RAPD. A specific band was confirmed by ISSR-5 primer in the 'Marguerite' cultivar strain of 'Zairai Shiro.' This primer ISSR-5 detected and identified certain specific band among the band pattern of 'Southern Elegance White,' this cultivar has cv. 'Early White' as the paternal parent. Similar polymorphic band pattern was confirmed by ISSR-PCR, in the breeding lines and the cultivars of the 'Marguerite' which have similarity in flower color, flowering type and share the hybrid parents.
Variabilities in karyotype and molecular ISSR inGonospermum fruticosum (C. Smith ex Link) Less., Argyranthemum coronopifolium (Willd.) Humphries and two strains of A. foeniculaceum (Willd.) Webb ex Schultz-Bip. (Asteraceae the tribe Anthemideae) collected in the Canary Islands ABSTRACT. Gonospermum fruticosum (C. Smith ex Link) Less, Argyranthemum coronopifolium (Willd.) Humphries and two strains of A. foeniculaceum (Willd.) Webb ex Schultz-Bipl all placed in the family Asteraceae, the tribe Anthemideae, were collected in Tenerife, the Canary Islands, Spanish Territory, Africa. They were analyzed in order to clarify their relationships and variabilities by karyotypes and molecular ISSR (inter simple sequence repeat). Gonospermum fruticosum and Argyranthemum coronopifolium studied showed the common karyotype different from that of two strains of A. foeniculaceum. Among the 54 ISSR bands obtained in the four species studied 46 were polymorphic, and 22 were species-specific. Gonospermum and Argyranthemum showed quite different band patterns from each other. Two strains of A. foeniculaceum showed different, large polymorphic patterns. Thus, the ISSR must be useful tool to isolate and evaluate intergeneric, interspecific, down to intraspecific genic differences, furthermore, introgressive hybridization within the members of the tribe Anthemideae, the Asteraceae.
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