Variabilities in karyotype and molecular ISSR in Gonospermum fruticosum (C. Smith ex Link) Less., Argyranthemum coronopifolium (Willd.) Humphries and two strains of A. foeniculaceum (Willd.) Webb ex Schultz-Bip. (Asteraceae the tribe Anthemideae) collected in the Canary Islands

Abstract

Variabilities in karyotype and molecular ISSR inGonospermum fruticosum (C. Smith ex Link) Less., Argyranthemum coronopifolium (Willd.) Humphries and two strains of A. foeniculaceum (Willd.) Webb ex Schultz-Bip. (Asteraceae the tribe Anthemideae) collected in the Canary Islands ABSTRACT. Gonospermum fruticosum (C. Smith ex Link) Less, Argyranthemum coronopifolium (Willd.) Humphries and two strains of A. foeniculaceum (Willd.) Webb ex Schultz-Bipl all placed in the family Asteraceae, the tribe Anthemideae, were collected in Tenerife, the Canary Islands, Spanish Territory, Africa. They were analyzed in order to clarify their relationships and variabilities by karyotypes and molecular ISSR (inter simple sequence repeat). Gonospermum fruticosum and Argyranthemum coronopifolium studied showed the common karyotype different from that of two strains of A. foeniculaceum. Among the 54 ISSR bands obtained in the four species studied 46 were polymorphic, and 22 were species-specific. Gonospermum and Argyranthemum showed quite different band patterns from each other. Two strains of A. foeniculaceum showed different, large polymorphic patterns. Thus, the ISSR must be useful tool to isolate and evaluate intergeneric, interspecific, down to intraspecific genic differences, furthermore, introgressive hybridization within the members of the tribe Anthemideae, the Asteraceae.

A molecular phylogenetic relationship of certain species of <i>Argyranthemum</i> found in the Canary Islands of Spain on the basis of the internal transcribed spacer (ITS)

“…Those seven Canary Islands were well isolated and way far from the continent where was covered by glaciers during the glacial epoch and thus, speciations in endemic species of Argyranthemum were well developed during their ice age. Several research papers on Argyranthemum have been published (Bramwell and Bramwell 1974;Humphries 1976;Bremer and Humphries 1993;Tatarenko et al 2013). In this study, the differentiations among the species of Argyranthemum located in Canary Islands was studied based on the information of internal transcribed spacer (ITS) regions.…”

Identifying, discriminating and isolating cultivars of &lsquo;Marguerites&rsquo; originated from <I>Argyranthemum frutescens</I> parentages and their intergeneric and interspecific hybridities by DNA markers amplified by RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat)&lowast;

“…Humphries originally collected in Anega Area, Tenerife, the Canary Islandand, two plants of A. foeniculaceum Webb ex Sch. (A and B) originally collected in Anega Area (Tatarenko et al 2013) were used for the present study. Not only with those species of Argyranthemum but also the other species of Argyranthemum could hybridize with each other.…”

“…Comparing those amplified polymorphisms with RAPD can be used for identifying cultivars as well as hybridities. The ISSR is amplified by using the sequence of an adjacent microsatellite region for a primer (Zietkiewicz et al 1994;Kondo et al 2003;Tatarenko et al 2013). Analyses of polymorphism with ISSR are partially inserted into the microsatellite region in the genome.…”