A B S T R A C TA total of 213 milk samples from clinically mastitic cattle cows were collected from different Governorates of Egypt and transferred in ice box as soon as possible to Bacteriological lab. in Animal Reproduction Research Institute (ARRI) in Giza Governorate (AL-Haram) for bacteriological examination of most important pathogens causing clinical mastitis with special references for isolation and strict identification of Salmonella species. All samples were collected during the period from December 2016 till July 2017 from governorates of Egypt. The bacteriological investigations revealed that 8 (3.7%) of Salmonella isolates were identified biochemically from all examined samples. Serological study showed that a total of 5 (2.3%) of Salmonella isolates were typed as Salmonella Typhimurium. Two strains of Salmonella Typhimurium were isolated singly in the rate of (0.93%) from all examined samples, also another two strains of Salmonella Typhimurium were isolated mixed with Staph aureus in the rate of (0.93%), meanwhile only one strain of same species was isolated mixed with E.coli in the rate of (0.47%). Cefiquinom and Enrofloxacin were sensitively in the rate of (100%), Ampicillin, Chloraphincol, Cloxacillin were resistance in the rate of (100 %) to Streptomycin and Amoxicillin. The molecular examination confirmed that all 5 examined serotyped strains were Salmonella Typhimurium. Virulence genes invA, hilA, avrA, were detected in examined sample by 100%, meanwhile sopE, ssaQ, and fimH genes were not detected by zero%. The objectives of the present study was to investigate the occurrence of Salmonella serotypes as well as to determine the frequency distribution of Six virulence genes (invA, hilA, avrA, ssaQ, sopE and fimH) in salmonella isolates from cattle clinical mastitic milk, in addition to determine the drugs of choice for treatment of most Salmonella strains causing cattle clinical mastitis.

Chronic Otitis Media (COM) is a long standing inflammation of the middle ear and mastoid, it is a common health problem. The evidence of microbiology in COM in different studies showed variable frequencies, but little studies showed the role of fungal colonization. This study aimed to identify the microbiology of cholesteatomatous COM concentrating on clarification of fungal colonization. A prospective study was done in otolaryngology department of Basrah Teaching Hospital from June 2018 till July 2019. This study was carried on a randomly collected data of 25 patients diagnosed as COM by a specific designed questionnaire including history and examination. The specimen was collected during mastoid surgery which included any cholesteatoma in the middle ear and mastoid and sent for bacteriological and mycological examination. Statistical study was done by using SPSS v.23 Bacteria were isolated in 84% of the cases in which Pseudomonas aeruginosa was the commonest one (33.3%). Fungi were isolated in 28% cases, in which the Aspergillosis was the commonest (19.3%). There is no recognizable pattern of significant association between the fungi and the reported complications or the stage of the disease. In conclusion, there is significant percentage of fungal colonization in COM with cholesteatoma so it may has a role in inflammatory response of perimatrix, but there is no clear mechanism of this interaction, so may need larger sample and longer duration of study with postoperative follow-up and trail of combined antibiotic-antimycotic treatment.

Background: Coronary Artery disease (CAD) is the leading cause of death globally. The classic presentation of worsening angina culminating acute coronary syndrome (ACS) is rare in younger patients but the first onset of angina that rapidly progresses to fully evolved myocardial infarction (MI) is often the case. The aim of this work was to study the short term outcome in patients undergoing primary percutaneous coronary intervention for acute ST elevation myocardial infarction. Methods: This was study a prospective, cross-sectional study carried out between July2020 to July 2021. The patients underwent coronary angiography at the National Heart Institute and cardiology department of Benha university hospitals. The patients were classified into two groups: -Group1: comprised 100 patients with coronary artery disease (CAD) aged <45 years old. Group2: comprised 100 patients with CAD aged ≥45 years old. Results: The rate of success of primary intervention was comparable between the two study groups 95% in young group vs 90% in older group. Conclusion: Acute STEMI in young Egyptians was predominantly observed in men, MACE was less frequent in Young age group.

This work aims to study the genetic characteristics of Staphylococcus aureus (S. aureus) strains isolated from 200 samples collected from humans (mouth, pharynx and hand) and food as poultry, milk and human food (cocked Liver and burgers sandwich). Genetic characteristics were evaluated epeningon resistance genes against some antibiotics that may be used for treatment of infected cases. A total of 23 isolates of S. aureus were identified from the collected 200 samples in an incidence rate of 11.5%. The identified strains were screened for resistance against 11 different antimicrobial agents. The strains showed a high level of resistance about (85-90%) to methicillin, penicillin; tobramycin, trimethoprim, sulfamethoxazone-trimethoprim and ciprofloxacin. Furthermore, moderate resistance to gentamycin, levofloxacin and lomfloxacin about (40-60%), while clindamycin and tetracycline antimicrobial agents were had a very low resistance, reaching (5-10%). The isolated S. aureus strains were monitored for the most important resistant genes the incidence rate of mecA, blaZ and tetK were (100%), while the aac(6'), aph(2") and norA were (45.5%) and (90.9%), respectively. This work revealed that about 70% of the isolated S.aureus strains were resistant to antibiotic drugs. Therefore, the miss, hub-hazard and uncontrolled use of antibiotics in veterinary medicine must be prevented.