In this review, we present an overview of the state of the art concerning the fundamental properties of electrode polarization (EP) of interest in the measurement of high conductivity samples and its implications for both dielectric (DS) and impedance spectroscopy (IS). Initially a detailed description of what constitutes EP is provided and the problems that it induces. Then, we review some of the more popular models that have been used to describe the physical phenomena behind the formation of the ionic double layer. Following this we shall enumerate the common strategies used historically to correct its influence on the measured signals in DS or in IS. Finally we also review recent attempts to employ fractal electrodes to bypass the effects of EP and to offer some physical explanation as to the limitations of their use.
A multi-layer micro-electrode structure has been developed for the selective manipulation and separation of bioparticles using travelling field dielectrophoresis effects. An important feature is that, in the separation process, the selected particles move in a stationary supporting fluid. Stationary suspensions of viable and non-viable yeast cells were used as a model system to demonstrate the general application of this device for the selective retention or transport of bioparticles in suspended mixtures. The efficiency of this process depends on the dielectric properties of the particles and their suspending medium, and is a sensitive function of the frequency of the travelling field. Apart from their use as particle separators, such micro-electrode devices are also envisaged to form integral components in the development of `biofactory on a chip' technology.
A dielectrophoresis (DEP) cell profiler, with concurrent FACS measurements, was used to monitor the morphological changes of Jurkat T-cells as they progressed through chemically induced apoptosis using etoposide. The cell 'physiometry' profiling technique measures the radius and the so-called DEP crossover frequency f(xo) of individual cells in a suspension, and this information was used to determine the effective plasma membrane capacitance of each cell. Control cells (n=526) exhibited a dynamic spread of f(xo) values, ranging from 50 to 250 kHz, and as apoptosis progressed over 6 h, the upper value for f(xo) progressively increased and extended beyond 500 kHz. This corresponded to a reduction in plasma membrane capacitance from 13.34 (+/-2.88) to 10.49 (+/-4.00) mF/m(2), and reflected a general smoothing of the membrane through loss of microvilli, for example. This is in broad agreement with previously reported studies of HL-60 cells undergoing apoptosis, but the authors' observation of a dynamic spread of f(xo) values does not agree with the earlier report that the f(xo) values for viable and apoptotic cells fall into two separable, relatively narrow, frequency bands. This has implications when devising protocols for the efficient DEP separation of viable, apoptotic and necrotic cells.
Dielectrophoresis has been used to enrich selected cell subpopulations in a mixed cell population by exploiting differential dielectric properties. Six-fold enrichment of stem cells expressing the CD34+ antigen has been achieved for bone marrow samples and peripheral blood, without the requirement for initial chemical treatment associated with immunoadsorption techniques.
In this paper, we report on the influence of D- and L-glucose on the dielectric properties of native shaped (biconcave) human erythrocytes using time domain dielectric spectroscopy. The dielectric spectra of biconcave cells were analysed using a modified form of the model originally reported for spheroid particle suspensions (Asami and Yonezawa 1995 Biochim. Biophys. Acta. 1245 317–24) The observed increase in the specific membrane capacitance of the biconcave erythrocytes was correlated with an increase in the concentration of D-glucose. In contrast, no associated correlation was found to changes in the membrane capacitance with increasing concentrations of L-glucose. A similar analysis of the dielectric response of osmotically swollen erythrocytes to changes in D-glucose concentration revealed a significantly different calculated specific cell membrane capacitance at elevated (>12 mM) D-glucose concentrations. The paper outlines and discusses the possible biochemical mechanisms that could be responsible for the measured dielectric properties of the erythrocyte membrane capacitances.
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