Enhancing traveling-wave dielectrophoresis with signal superposition

Pethig, Ronald; Talary, Mark S.; Lee, R.S.

doi:10.1109/memb.2003.1266046

Cited by 100 publications

(78 citation statements)

References 16 publications

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“…1,2 The fields employed for such work are generated using electrodes such as planar linear interdigitated arrays 3,4,1 or two parallel arrays. [5][6][7]2,8 The electrodes in these arrays are driven by sinusoidal quadrature-phase voltages.…”

Section: Introductionmentioning

confidence: 99%

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Dielectrophoretic spectra of translational velocity and critical frequency for a spheroid in traveling electric field

et al. 2010

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An analysis has been made of the dielectrophoretic ͑DEP͒ forces acting on a spheroidal particle in a traveling alternating electric field. The traveling field can be generated by application of alternating current signals to an octapair electrode array arranged in phase quadrature sequence. The frequency dependent force can be resolved into two orthogonal forces that are determined by the real and the imaginary parts of the Clausius-Mossotti factor. The former is determined by the gradient in the electric field and directs the particle either toward or away from the tip of the electrodes in the electrode array. The force determined by the imaginary component is in a direction along the track of the octapair interdigitated electrode array. The DEP forces are related to the dielectric properties of the particle. Experiments were conducted to determine the DEP forces in such an electrode arrangement using yeast cells ͑Saccharomyces cervisiate TISTR 5088͒ with media of various conductivities. Experimental data are presented for both viable and nonviable cells. The dielectric properties so obtained were similar to those previously reported in literature using other DEP techniques.

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Section: Introductionmentioning

confidence: 99%

“…The phase sequence addressing employed has been described by many investigators. 5,7,3,4,1,8 The two parallel electrode arrays offer advantages for cell manipulation and positioning. The methods allow cells to move either toward the electrode tips or along the electrode track simultaneously as a result of two orthogonal forces.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic spectra of translational velocity and critical frequency for a spheroid in traveling electric field

et al. 2010

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“…Similarly to trapping cells with an electric field using a micropatterned electrode, electrical charge may affect the cells. 17,18 It may also be of a concern that preventing cells to be naturally free-floating eventually results in changed growth kinetics. To study the effect of the charged surface on growth kinetics, we monitored groups of S. platensis filaments grown under 3 different conditions (10 groups each, mean 6 SD of initial filament number ¼ 9.8 6 1.4): (1) in wells with the positively charged based, (2) in wells with normal glass surface base, and (3) in static bulk culture (500 ml flask).…”

Section: Effect Of the Electrostatic Charged To Cell Growth Kineticsmentioning

confidence: 99%

“…The electrical field may also affect cell proliferation. 17,18 Cell trapping based on suction, 15,19 channel constriction 20 or microcage 21 may be considered a less costly alternative. Their great difficulty is that there is not a single universal trap design of this kind that can handle multiple types of algal species.…”

Section: Introductionmentioning

confidence: 99%

An electrostatic microwell–based biochip for phytoplanktonic cell trapping

et al. 2014

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A simple microwell-based microfluidic chip for microalgal cells trapping was fabricated. An electrostatic cell trapping mechanism, enabled by a positively charged glass surface, was used. The chip was capable of capturing multiple algal cell types. In the case of filamentous Spirulina platensis, we observed single filament occupancy of up to $30% available wells, as high as some previously proposed methods. Captured filaments were not of any preferential size, suggesting well randomized cell trapping. It was found that the electrostatic attraction did not affect the cell growth. Total replacement of liquid inside the wells could be achieved by pumping new solutions via the inlet, making single cell experiments in controlled chemical conditions possible. After the top layer of the chip was removed, cells in the wells could be simply transferred using a micropipette, turning the chip into a platform for strain selection. V C 2014 AIP Publishing LLC. [http://dx

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“…Traveling wave DEP is the method of changing the phase of the applied electric field 53,54 in order to achieve the gradient of the electric field between parallel electrodes. Insulating DEP ͑iDEP͒ consists of generating a gradient of an electric field in a capacitor-like structure using a nonhomogenous dielectric medium.…”

Section: Dielectrophoretic "Dep… Trappingmentioning

confidence: 99%

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

Choudhury

Iliescu

et al. 2011

Biomicrofluidics

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There are a plethora of approaches to construct microtissues as building blocks for the repair and regeneration of larger and complex tissues. Here we focus on various physical and chemical trapping methods for engineering three-dimensional microtissue constructs in microfluidic systems that recapitulate the in vivo tissue microstructures and functions. Advances in these in vitro tissue models have enabled various applications, including drug screening, disease or injury models, and cellbased biosensors. The future would see strides toward the mesoscale control of even finer tissue microstructures and the scaling of various designs for high throughput applications. These tools and knowledge will establish the foundation for precision engineering of complex tissues of the internal organs for biomedical applications.

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Enhancing traveling-wave dielectrophoresis with signal superposition

Cited by 100 publications

References 16 publications

Dielectrophoretic spectra of translational velocity and critical frequency for a spheroid in traveling electric field

Dielectrophoretic spectra of translational velocity and critical frequency for a spheroid in traveling electric field

An electrostatic microwell–based biochip for phytoplanktonic cell trapping

Exploitation of physical and chemical constraints for three-dimensional microtissue construction in microfluidics

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