Separation of viable and non-viable yeast using dielectrophoresis

Markx, Gerard H.; Talary, Mark S.; Pethig, Ronald

doi:10.1016/0168-1656(94)90117-1

Cited by 262 publications

(185 citation statements)

References 15 publications

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“…For example, work has demonstrated that DEP can be used to examine the effect of drugs on cells, such as the effect of nystatin on erythrocytes (5) or the response of neutrophils to activation by chemotactic factors (6). Since populations of particles may experience forces acting in different directions within specific frequency windows, separation has been demonstrated for mixtures of viable and non-viable yeast cells (7), and CD34+ cells from bone marrow, which can be separated from human blood (8). Other demonstrations of cell separation have been made in separating breast cancer cells from blood, erythrocytes with and without malarial parasite infection, B-and T-lymphocytes, and different types of viruses in solution (9)(10)(11)(12).…”

Section: By Exploiting the Fact That Different Particles May Experienmentioning

confidence: 99%

Dielectrophoresis-Activated Multiwell Plate for Label-Free High-Throughput Drug Assessment

et al. 2008

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Dielectrophoresis (DEP) offers many advantages over conventional cell assays such as flow cytometry and patch clamp techniques for assessing cell electrophysiology as a marker for cancer studies and drug interaction assessment. However, despite the advantages offered by DEP analysis, uptake has been low, remaining largely in the academic arena, due to the process of analysis being time-consuming, laborious, and ultimately allowing only serial analysis on small numbers of cells. In this paper we describe a new method of performing DEP analysis based on laminate manufacturing methods. These use a three-dimensional “well” structure, similar in size and pitch to conventional microtiter well plates, but offer electrodes along the inner surface to allow easy measurement of cell properties through the whole population. The result can then be determined rapidly using a conventional well-plate reader. The nature of the device means that many electrodes, each containing a separate sample, can be tested in parallel, while the mode of observation means that analysis can be combined with simultaneous measurement of conventional fluorimetric well-based assays. Here we benchmark the device against standard DEP assays, then show how such a device can be used to (a) rapidly determine the effects both of ion channel blockers on cancer cells and antibiotics on bacteria and (b) determine the properties of multiple subpopulations of cells within a well simultaneously.

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Section: By Exploiting the Fact That Different Particles May Experienmentioning

confidence: 99%

Dielectrophoresis-Activated Multiwell Plate for Label-Free High-Throughput Drug Assessment

et al. 2008

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“…12 Another attractive feature of the DEP force is that cells can be either attracted (positive DEP [pDEP]) or repelled (negative DEP [nDEP]) by changing the conductivity difference between the cell and the surrounding medium. Owing to these features, the DEP force has been widely employed for cell separation, 15 electroinjection, 16 electrofusion 17 and electroporation. 18 Despite these advantages and developments, however, micro-scale electrodes that were used for generating the DEP force required complex photolithography and microfabrication processes that must be performed in specialized cleanroom facilities.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

et al. 2014

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Gene transfection is an important technology for various biological applications. The exogenous DNA is commonly delivered into cells by using a strong electrical field to form transient pores in cellular membranes. However, the high voltage required in this electroporation process may cause cell damage.In this study, a dielectrophoretically-assisted electroporation was developed by using light-activated virtual microelectrodes in a new microfluidic platform. The DNA electrotransfection used a low applied voltage and an alternating current to enable electroporation and transfection. Single or triple fluorescencecarrying plasmids were effectively transfected into various types of mammalian cells, and the fluorescent proteins were successfully expressed in live transfected cells. Moreover, the multi-triangle optical pattern that was projected onto a photoconductive layer to generate localized non-uniform virtual electric fields was found to have high transfection efficiency. The developed dielectrophoretically-assisted electroporation platform may provide a simpler system for gene transfection and could be widely applied in many biotechnological fields.

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“…Depending on the particle polarisability compared with the suspending medium, the particle moves either towards the location with the greatest electric field gradient (positive DEP), or location away from the highest electric field gradient (negative DEP) (Pohl, 1978). DEPbased techniques have been successfully used for many biological applications to date, such as separations of viable and nonviable yeast cells (Huang et al, 1992;Markx et al, 1994), separation of live and heat-treated listeria bacteria (Li and Bashir, 2002), isolation and detection of sparse cancer cells, concentration of cells from dilute suspensions, and trapping and positioning of individual cells for characterization (Wang et al, 1997). Among these, the simplest method of practical dielectrophoretic separation is that of flow separation by using microfabricated interdigitated electrode array at the bottom of the microfluidic devices (Hughes, 2002).…”

Section: Introductionmentioning

confidence: 99%

On the Design and Optimization of Micro-Fluidic Dielectrophoretic Devices: A Dynamic Simulation Study

Bashir

2004

Biomedical Microdevices

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Abstract. Microfabricated interdigitated electrode array is a convenient form of electrode geometry for dielectrophoretic trapping of biological particles within micro-fluidic biochips. We have previously reported experimental results and finite element modeling of the holding forces for both positive and negative dielectrophoretic traps on microfabricated interdigitated electrodes within a microfluidic biochip fabricated in silicon with a 12 µm deep chamber and anodic-bonded glass cover. Based on these prior studies, we present in this paper a dynamic study to investigate the stopping capability of dielectrophoretic devices with limited electrode teeth. Simulation results on the issues of design and optimization of the dielectrophoretic devices are also presented and discussed in detail. Simulation results show that the maximum particle stopping distance in a specific device is very sensitive to the chamber height due to the near-electrode nature of DEP force. The relationship between maximum stopping distance and the applied voltage is presented, and the electrode spacing is found to be important in designing the electrode geometry. The spacing should be no less than the chamber height in order to efficiently capture the particles in a relatively short range at a given applied voltage and flow rate.

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Separation of viable and non-viable yeast using dielectrophoresis

Cited by 262 publications

References 15 publications

Dielectrophoresis-Activated Multiwell Plate for Label-Free High-Throughput Drug Assessment

Dielectrophoresis-Activated Multiwell Plate for Label-Free High-Throughput Drug Assessment

Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

On the Design and Optimization of Micro-Fluidic Dielectrophoretic Devices: A Dynamic Simulation Study

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