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Background-In many digestive diseases the intestinal barrier is weakened by the release of proinflammatory cytokines, including tumour necrosis factor-a (TNFa). Aim-To investigate the protective effect of apical diosmectite on the intestinal dysfunction induced by the proinflammatory cytokine TNFa. Methods-Filter grown monolayers of the intestinal celi line HT29-19A were incubated for 48 hours in basal medium containing 10 ng/ml TNFt and 5 U/ml interferon-y (IFNy). Next, 1, 10, or 100 mg/ml diosmectite was placed in the apical medium for one hour.

Neuroprotective drugs such as Ginkgo biloba extract (EGb 761) could prevent the ischemia-induced impairment of the Na,K-ATPase activity. In this study, Na,K-ATPase activity and expression, contents in fatty acids and malondialdehyde, an index of lipoperoxidation, were compared in the ipsilateral (ischemic) and the contralateral (unlesioned) cortices after 1 h of unilateral focal cortices cerebral ischemia in the mouse. EGb 761 (110 mg/kg) was administered daily to half of the animals for 10 days before ischemia. Ischemia significantly reduced Na,K-ATPase activity by about 40% and increased malondialdehyde content; EGb 761 pretreatment abolished these effects. The free radical scavenger properties of EGb 761 are a potential mechanism by which Na,K-ATPase injury and lipoperoxidation are prevented.

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