We are interested in developing monoclonal antibodies (MoAbs) that recognize specific cell types in the lung of BALB/c mice. Normal mouse lung homogenate was used to immunize F344 rats and hybnidomas were produced by fusion of rat spleen cells with mouse mycloma SP 2/0. Two hybndomas were selected which produced MoAbs active in immunohistochemistry of lung cells. MoAb 273-34A and 41 1-201B both show extensive peroxidase staining of capillary endothelial cells within alveolar walls of lungs at the light microscopic level. To demonstrate cell specificity, immunoelectron microscopy with gold-labeled antibody was performed. Lightly fixed lungs were frozen and thinsectioned before staining with MoAb and 5-nm gold parti-I Sponsored jointly by NIH Grant No.
SUMMARY
A simple and inexpensive slam freezing device has been constructed that allows consistently good rapid freezing of uncryoprotected tissue. The device employs a dry liquid‐nitrogen‐cooled copper surface and a slamming plunger designed to provide rapid and uniform tissue to metal contact.
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