Rat monoclonal antibodies to mouse lung components for analysis of fibrosis

Kennel, Stephen J.; Hotchkiss, Jon A.; Rorvik, Marie C.; Allison, David P.; Foote, Linda J.

doi:10.1016/0014-4800(87)90012-8

Cited by 19 publications

(7 citation statements)

References 23 publications

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“…Endothelial cells were identified using a number of specific markers, that is, von Willebrand factor (vWF) (8,12,15), PECAM (CD31) (1, 23), thrombomodulin (10,16), LuECAM-1 (354, Dil-Ac-LDL uptake (7,11,31), and binding of the lectin, Grifonia simpl$oLia-l (BS-1 B4) (17). Polyclonal antiserum against human vWF was obtained from DAKO (Carpineria, CA).…”

Section: Cell Identificationmentioning

confidence: 99%

“…Monoclonal antibody directed against murine PECAM-1 was obtained from Dr. S. Bogen (Boston University, Boston, MA). Monoclonal antibody against murine thrombomodulin (10,16) For immunohistochemical studies, cells were fixed with 100% -20°C methanol (10 min), then incubated in PBS containing 1 % bovine serum albumin. After blocking 10 min in PBS containing 10 pg/ml nonimmune second antibody, cells were incubated 4 hr at 4°C with 10 pg/ml of the first antibody, washed, and incubated 30 rnin with biotinylated second antibody, followed by incubation with a 1 : 100 dilution of Streptavidin-FITC.…”

Section: Cell Identificationmentioning

confidence: 99%

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Activation‐Dependent Isolation and Culture of Murine Pulmonary Microvascular Endothelium

Gerritsen¹,

Shen²,

McHugh³

et al. 1995

Microcirculation

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Section: Cell Identificationmentioning

confidence: 99%

Section: Cell Identificationmentioning

confidence: 99%

Activation‐Dependent Isolation and Culture of Murine Pulmonary Microvascular Endothelium

Gerritsen¹,

Shen²,

McHugh³

et al. 1995

Microcirculation

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“…Two MAbs generated against lung homogenates react with a protein (pl12) that has restricted expression to lung capillary endothelium [87]. Administration of these MAbs into mice is reported to cause increased lung colonization by BI6-F10 melanoma cells [88].…”

Section: Antigenic Heterogeneity Of Endotheliummentioning

confidence: 99%

Microvascular endothelial cell heterogeneity: Interactions with leukocytes and tumor cells

Belloni

Tressler

1990

Cancer Metast Rev

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Endothelium constitutes a highly specialized organ that lines the vascular system and lymphatic channels. This organ is a complex network of arteries, veins, and microvessels that differ in size, structure, and function. The unique and strategic location imposes functional demands on the endothelium that are far greater than just being a passive barrier. Endothelial cells have the ability to differentiate both in structure and function in response to the needs of diverse tissue environments, making this organ extremely heterogeneous. Although vascular endothelial cells share certain common properties, they differ in regard to structure, antigenic and cell surface determinants, adhesion molecules, and metabolic function. The unique cell surface profiles expressed by endothelial cells in different tissue locations can be recognized by specific populations of circulating leukocytes or tumor cells, which contribute to their arrest and invasion patterns. This article attempts to review our current understanding of endothelial cell heterogeneity and its significance to patterns of leukocyte and tumor cell trafficking.

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“…MoAbs 273-34A and 411-201B are rat subclass IgG2a, the binding specificities of which have been previously described (Kennel et al 1987, Rorvik et al 1988) Rat MoAb 135-14 (subclass IgGza), used as a negative control, has no known binding specificity.…”

Section: Methodsmentioning

confidence: 99%

Thrombomodulin distribution during murine development

Ford

Wilkinson

Kennel

1993

Roux's Arch Dev Biol

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Thrombomodulin is a transmembrane glycoprotein involved in the regulation of clot formation. Previous work has shown that in the adult mouse, thrombomodulin expression is primarily located on the luminal surface of endothelial cells; whereas during development, thrombomodulin has been localized to the placental parietal endoderm, the neuroepithelium, head mesenchyme, lung bud, and atrium of Day 10.5 post coitum embryos. We examined the expression of thrombomodulin in the adult mouse and throughout murine development from Day 7.5 to Day. 18.5 post coitum. The high expression of thrombomodulin in the lung bud, developing gonads, leptomeninges, and the parietal endoderm in comparison to the expression levels in the adult organs suggest that thrombomodulin may play an important role in development.The submitted manuscript has been authored by a contractor of the U.S. Government under contract No. DE-AC05-840R21400. Accordingly, the U.S. Government retains nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for US Government purposes.

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Rat monoclonal antibodies to mouse lung components for analysis of fibrosis

Cited by 19 publications

References 23 publications

Activation‐Dependent Isolation and Culture of Murine Pulmonary Microvascular Endothelium

Activation‐Dependent Isolation and Culture of Murine Pulmonary Microvascular Endothelium

Microvascular endothelial cell heterogeneity: Interactions with leukocytes and tumor cells

Thrombomodulin distribution during murine development

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