Thrombomodulin distribution during murine development

Ford, Virginia A.; Wilkinson, John E.; Kennel, Stephen J.

doi:10.1007/bf00188735

Cited by 18 publications

(10 citation statements)

References 33 publications

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“…By this hypothesis the absence of Thrombomodulin from blood vessel endothelium may cause excessive activation of the embryonic blood coagulation system [17]. We cannot state at present the exact meaning of a reduced expression of Thrombomodulin in placental tissue.…”

Section: Discussionmentioning

confidence: 99%

Placental thrombomodulin expression in recurrent miscarriage

Stortoni

Cecati

Giannubilo

et al. 2010

Reprod Biol Endocrinol

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BackgroundEarly pregnancy loss can be associated with trophoblast insufficiency and coagulation defects. Thrombomodulin is an endothelial-associated anticoagulant protein involved in the control of hemostasis and inflammation at the vascular beds and it's also a cofactor of the protein C anticoagulant pathway.DiscussionWe evaluate the Thrombomodulin expression in placental tissue from spontaneous recurrent miscarriage and voluntary abortion as controls. Thrombomodulin mRNA was determined using real-time quantitative polymerase chain reaction. Reduced expression levels of thrombomodulin were found in recurrent miscarriage group compared to controls (1.82-fold of reduction), that corresponds to a reduction of 45% (from control group Delta CT) of thrombomodulin expression in spontaneous miscarriage group respect the control groups.SummaryWe cannot state at present the exact meaning of a reduced expression of Thrombomodulin in placental tissue. Further studies are needed to elucidate the biological pathway of this important factor in the physiopathology of the trophoblast and in reproductive biology.

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Section: Discussionmentioning

confidence: 99%

Placental thrombomodulin expression in recurrent miscarriage

Stortoni

Cecati

Giannubilo

et al. 2010

Reprod Biol Endocrinol

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“…Abolishing Tie2 function by knocking out the receptor (3,13) or its recently identified activating ligand (2,16) results in embryonic lethality in mice between embryonic days E9. 5 and E10.5 (3,13,16). This knowledge indicates the importance of understanding the mechanisms of Tie2 gene regulation during (patho)physiological processes.…”

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confidence: 85%

Targeting theHprtlocus in mice reveals differential regulation ofTie2gene expression in the endothelium

Evans

Hatzopoulos²,

Aird

et al. 2000

Physiological Genomics

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To study the in vivo expression of the murine Tie2 gene, we have targeted the hypoxanthine phosphoribosyltransferase (Hprt) gene locus to generate two single-copy transgenic mice: T1, containing the 2,100-bp Tie2 promoter upstream from the beta-galactosidase (LacZ) gene, and T5, which also included an enhancing element originating from the first intron of the Tie2 gene. Comparing T1 and T5 embryos at day E10.5 revealed differential endothelial cell-specific expression of LacZ, whereas colocalization analyses showed that the expression was confined to endothelial cells. Moderate reporter gene activity was observed in the brain and kidney of T1 adults, whereas extensive LacZ gene expression was seen in the vasculature of most organs of the T5 adults. This study demonstrates the feasibility of targeting the Hprt locus with endothelial cell-specific sequences to analyze the spatial-temporal expression of transgenes. Of particular importance is the observation that the analysis of a single transgene copy in a defined locus allows for an accurate and rapid comparison of transcriptional activity among regulatory DNA sequences.

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“…compound (Allegiance Health Care Corp., Omaha, NE). Formalin-fixed, paraffin-embedded sections of all wounds were stained with Masson's trichrome or analyzed for murine thrombomodulin by immunoperoxidase staining, using the monoclonal antibody 273-34A 21 (generously provided by Dr. Stephen J. Kennel, Oak Ridge National Laboratory, Oak Ridge, TN) as described previously. 14 To detect ␤-galactosidase activity, frozen sections were fixed for 5 minutes in acetone and washed two times with phosphate-buffered saline.…”

Section: Histological Analysismentioning

confidence: 99%

Expression of Thrombomodulin and Consequences of Thrombomodulin Deficiency during Healing of Cutaneous Wounds

Peterson

Rayburn

Lager

et al. 1999

The American Journal of Pathology

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Thrombomodulin is a cell surface anticoagulant that is expressed by endothelial cells and epidermal keratinocytes. Using immunohistochemistry , we examined thrombomodulin expression during healing of partial-thickness wounds in human skin and fullthickness wounds in mouse skin. We also examined thrombomodulin expression and wound healing in heterozygous thrombomodulin-deficient mice , compound heterozygous mice that have <1% of normal thrombomodulin anticoagulant activity , and chimeric mice derived from homozygous thrombomodulin-deficient embryonic stem cells. In both human and murine wounds , thrombomodulin was absent in keratinocytes at the leading edge of the neoepidermis , but it was expressed strongly by stratifying keratinocytes within the neoepidermis. No differences in rate or extent of reepithelialization were observed between wild-type and thrombomodulin-deficient mice. In chimeric mice , both thrombomodulin-positive and thrombomodulin-negative keratinocytes were detected within the neoepidermis. Compared with wild-type mice , heterozygous and compound heterozygous thrombomodulin-deficient mice exhibited foci of increased collagen deposition in the wound matrix. These findings demonstrate that expression of thrombomodulin in keratinocytes is regulated during cutaneous wound healing. Severe deficiency of thrombomodulin anticoagulant activity does not appear to alter reepithelialization but may influence collagen production by fibroblasts in the wound matrix. (Am J Pathol 1999, 155:1569 -1575)Thrombomodulin is an anticoagulant glycoprotein that is expressed on the surface of vascular endothelial cells and epidermal keratinocytes.

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Thrombomodulin distribution during murine development

Cited by 18 publications

References 33 publications

Placental thrombomodulin expression in recurrent miscarriage

Placental thrombomodulin expression in recurrent miscarriage

Targeting theHprtlocus in mice reveals differential regulation ofTie2gene expression in the endothelium

Expression of Thrombomodulin and Consequences of Thrombomodulin Deficiency during Healing of Cutaneous Wounds

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