Melipona bees are remarkable due to the high contrast in heterochromatin amounts, making this group interesting for studying repetitive DNA amplification. Here, we performed the first efforts for the chromosomal localization of different repetitive DNAs in M. (Michmelia) scutellaris and tested for unique or multiple heterochromatin amplification in Melipona subgenera. Our data revealed enrichment of repetitive DNAs in chromosomal heterochromatic arms demonstrated by C 0 t-DNA and DOP-PCR probe hybridization, although microsatellites and multigene families were located at terminal euchromatic regions. Analysis using C 0 t-DNA probe from M. scutellaris showed positive hybridization only in Michmelia species, suggesting monophyletic amplification and sharing of heterochromatin sequences between species. However, the subgenus Melikerria , with a high amount of heterochromatin, probably underwent independent heterochromatin amplification or experienced sequence modification.
Repetitive DNAs constitute a significant part of the genomes and are used as cytogenetic markers for the understanding of chromosome evolution. Bees of the genus Melipona presents conserved chromosome number but high variability concerning heterochromatin distribution, dividing the genus into two groups: (i) species with low quantity of heterochromatin and (ii) species with high amount of heterochromatin. Here aiming the understanding of repetitive DNAs chromosomal organization in Melipona we mapped through fluorescence in situ hybridization (FISH) some repetitive DNAs in M. scutellaris. For this, FISH were performed with the amplification products of the 18S rDNA, U2 snDNA and DOP-PCR, as well as the product of the C0t fraction and different microsatellites. The mitotic chromosomes used in FISH were obtained by squashing the brain ganglia, and in this material, being performed also the C-band and CMA/DAPI. The distribution of the C0t fraction in other species of Melipona was verified with membrane hybridization. Chromosome number of n = 9 and 2n = 18 (male and female, respectively) was confirmed and a large amount of heterochromatin was observed along the chromosomes, except at the terminals that were characterized as euchromatic and only rich in different microsatellites. The heterochromatic regions were observed with richness in A + T and C + G. The FISH experiments showed signals from the 18S rDNA and U2 snDNA probes on distinct chromosomes. This demonstrates that these multigenic families followed different evolutionary paths in the chromosome evolution of the species. The DOP-PCR and C0t fraction showed signs of hybridization in the heterochromatic regions, and the C0t fraction of M. scutellaris was also observed in the genomes of M. rufiventris, M. rufiventris rufiventris and M. seminigra, evidencing the dispersion of similar repetitive elements in genomes of these three species. Thus, it was possible to characterize the karyotype of M. scutellaris with different cytogenetic markers. In addition, the results with membrane hybridization reinforce the hypothesis of the repetitive DNA sharing among nearby species, suggesting that there are different repetitive elements present in the genome of the genus Melipona.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.