Repetitive DNAs in Melipona scutellaris (Hymenoptera: Apidae: Meliponidae): chromosomal distribution and test of multiple heterochromatin amplification in the genus

Piccoli, Mariani Cristina Alves; Bardella, Vanessa Bellini; Cabral-de-Mello, Diogo Cavalcanti

doi:10.1007/s13592-018-0577-z

Cited by 31 publications

(21 citation statements)

References 42 publications

(44 reference statements)

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“…Microsatellite hybridization was observed in the DAPI-negative portion corresponding to euchromatin. Similar results have been obtained in other Meliponini studies [Ferreira, 2015;Piccoli et al, 2018;Travenzoli et al, 2019b]. The microsatellite probes bound and marked the chromosomes in all species tested ( Fig.…”

Section: Resultssupporting

confidence: 90%

“…Considering that heterochromatin in Meliponini arose at different times [Cunha et al, 2018;Piccoli et al, 2018;Pereira, 2018] and that chromatin with intermediate staining was observed only in species with centromeric heterochromatin, this "new class" of chromatin can indicate the beginning of the process of heterochromatinization in these species, and will result in the formation of completely heterochromatic arms as seen in high frequency in the Meliponini. Therefore, the verification of the existence of a new class of chromatin with intermediate staining intensity, between that of the euchromatin and heterochromatin, demonstrates a possible path of how karyotype evolution has taken place in the tribe Meliponini.…”

Section: Resultsmentioning

confidence: 99%

“…One possible explanation for the intermediate staining is that these are regions of heterochromatin formation that is involved in more than just the stabilization of the telomeric region, as proposed in Imai et al [1988]. Recent studies have shown that heterochromatin emerged at different times during the evolution of Meliponini species, indicating that its formation is a continuous and recurrent process [Cunha et al, 2018;Piccoli et al, 2018]. Recent developments in the field of cytogenetics include new techniques for studying chromosomes and new software for analyzing images, both leading to the investigation of chromosome banding patterns with improved depth and reliability.…”

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confidence: 97%

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Different Levels of Chromatin Condensation in Partamona chapadicola and Partamona nhambiquara (Hymenoptera, Apidae)

Lopes

Travenzoli

Fernandes

et al. 2020

Cytogenet Genome Res

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Studies in several organisms have contributed to the understanding of heterochromatin and its biological importance. In bees of the tribe Meliponini, the presence of chromosomes with totally heterochromatic arms has been attributed to the mechanism of karyotype evolution in which this group accumulated heterochromatin to maintain telomere stability after centric fission events. In the present study, the use of classical and molecular cytogenetic techniques as well as automated image analysis software for the description of the karyotypes of Partamonachapadicola and P. nhambiquara bee species revealed variability in the compaction and patterns of chromatin structure. Although both species have the same chromosome number as other species in the genus Partamona (2n = 34), C-banding and image analyses indicated the existence of chromosomes with 3 regions of different staining intensities, suggesting a chromatin structure with distinct patterns and characteristics. Repetitive DNA probes hybridized only in the euchromatic regions, whereas the regions with intermediate staining intensity did not show any hybridization signals. This suggests that these regions present features more similar to heterochromatin. Evidence of the existence of a chromatin class with intermediate condensation compared to euchromatin and heterochromatin indicates a potential mechanism for heterochromatin amplification and demonstrates the need for further studies on this topic. This previously unrecognized class of chromatin should be taken into account in the study of all Meliponini chromosomes.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 97%

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Different Levels of Chromatin Condensation in Partamona chapadicola and Partamona nhambiquara (Hymenoptera, Apidae)

Lopes

Travenzoli

Fernandes

et al. 2020

Cytogenet Genome Res

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“…3b, d), as already reported for the two colonies analysed by Rocha et al (2007). The presence of a unique autosome pair with a nucleolus organizer in M.quinquefasciata corroborated previous reports about the location of the rDNA clusters in other Melipona species, independent of the technique used (Ag-NOR impregnation, CMA 3 staining or FISH; Rocha et al 2002, Brito et al 2003, Lopes et al 2011, Cunha et al 2018, Piccoli et al 2018). This seemed to be the most frequent pattern found in other Meliponini genera (Brito-Ribon et al 1999, Rocha et al 2003, Krinski et al 2010), although the presence of multiple rDNA clusters has also been described (Rocha et al 2003, Brito et al 2005, Duarte et al 2009, Martins et al 2009, Godoy et al 2013).…”

Section: Resultssupporting

confidence: 88%

Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes

Silva

Rocha

Pompolo

et al. 2018

CCG

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Stingless bees are distributed widely in the tropics, where they are major pollinators of several plant species. In this study, the karyotype of Meliponaquinquefasciata Lepeletier, 1836 was analysed, with emphasis on the presence of B chromosomes. Post-defecating larvae were analysed using Giemsa staining, the C-banding technique, sequential staining with fluorochromes, and FISH. The chromosome number ranged from 2n = 18 to 22 (females) and from n = 9 to 13 (males) due to the presence of 0–4 B chromosomes. This result demonstrates that M.quinquefasciata has the same chromosomal number as other Melipona Illiger, 1806 species. Considering the A complement, heterochromatin was located only in the pericentromeric region of pair 1. Staining with chromomycin A3 (CMA3) and labelling with rDNA probe, indicated that this region corresponded to the nucleolus organising region. The B chromosomes of M.quinquefasciata could be found in individuals from different localities, they were completely heterochromatic (C-banding) and uniformly stained by 4’,6-diamidino-2-phenylindole (DAPI). Variations in the number of B chromosomes were detected between cells of the same individual, between individuals of the same colony, and between colonies from different localities.

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“…Cytogenetic analyses, in particular, are an important tool for understanding the macro-scale genomic organization of different any species. These analyses comprise descriptions of chromosome number [11], [2], [9], heterochromatin distribution patterns [12], characterization of AT and CG rich regions [13], [12], localization of 18S ribosomal genes [14], [12], mapping of repetitive DNA sequences [15], and inferences of karyotype evolution [10–11].…”

Section: Introductionmentioning

confidence: 99%

The evolution of haploid chromosome numbers in Meliponini

et al. 2019

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It is thought that two evolutionary mechanisms gave rise to chromosomal variation in bees: the first one points to polyploidy as the main cause of chromosomal evolution, while the second, Minimum Interaction Theory (MIT), is more frequently used to explain chromosomal changes in Meliponini and suggests that centric fission is responsible for variations in karyotype. However, differences in chromosome number between Meliponini and its sister taxa and in the karyotype patterns of the Melipona genus cannot be explained by MIT, suggesting that other events were involved in chromosomal evolution. Thus, we assembled cytogenetical and molecular information to reconstruct an ancestral chromosome number for Meliponini and its sister group, Bombini, and propose a hypothesis to explain the evolutionary pathways underpinning chromosomal changes in Meliponini. We hypothesize that the common ancestor shared by the Meliponini and Bombini tribes possessed a chromosome number of n = 18. The karyotype with n = 17 chromosomes was maintained in Meliponini, and variations of haploid numbers possibly originated through additional Robertsonian fissions and fusions. Thus, the low chromosome number would not be an ancestral condition, as predicted by MIT. We then conclude that Robertsonian fission and fusions are unlikely to be the cause of chromosomal rearrangements that originated the current karyotypes in Meliponini.

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Repetitive DNAs in Melipona scutellaris (Hymenoptera: Apidae: Meliponidae): chromosomal distribution and test of multiple heterochromatin amplification in the genus

Cited by 31 publications

References 42 publications

Different Levels of Chromatin Condensation in <b><i>Partamona chapadicola</i></b> and <b><i>Partamona nhambiquara</i></b> (Hymenoptera, Apidae)

Different Levels of Chromatin Condensation in <b><i>Partamona chapadicola</i></b> and <b><i>Partamona nhambiquara</i></b> (Hymenoptera, Apidae)

Karyotypic description of the stingless bee Melipona quinquefasciata Lepeletier, 1836 (Hymenoptera, Meliponini) with emphasis on the presence of B chromosomes

The evolution of haploid chromosome numbers in Meliponini

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