The larvae of scarab beetles, known as "white grubs" and belonging to the genera Phyllophaga and Anomala (Coleoptera: Scarabaeidae), are regarded as soil-dwelling pests in Mexico. During a survey conducted to find pathogenic bacteria with the potential to control scarab larvae, a native Serratia sp. (strain Mor4.1) was isolated from a dead third-instar Phyllophaga blanchardi larva collected from a cornfield in Tres Marías, Morelos, Mexico. Oral bioassays using healthy P. blanchardi larvae fed with the Mor4.1 isolate showed that this strain was able to cause an antifeeding effect and a significant loss of weight. Mortality was observed for P. blanchardi, P. trichodes, and P. obsoleta in a multidose experiment. The Mor4.1 isolate also caused 100% mortality 24 h after intracoelomic inoculation of the larvae of P. blanchardi, P. ravida, Anomala donovani and the lepidopteran insect Manduca sexta. Oral and injection bioassays were performed with concentrated culture broths of the Mor4.1 isolate to search for disease symptoms and mortality caused by extracellular proteins. The results have shown that Mor4.1 broths produce significant antifeeding effects and mortality. Mor4.1 broths treated with proteinase K lost the ability to cause disease symptoms and mortality, in both the oral and the injection bioassays, suggesting the involvement of toxic proteins in the disease. The Mor4.1 isolate was identified as a putative Serratia entomophila Mor4.1 strain based on numerical taxonomy and phylogenetic analyses done with the 16S rRNA gene sequence. The potential of S. entomophila Mor4.1 and its toxins to be used in an integrated pest management program is discussed.

Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology.

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We evaluated the impact of bacterial rhabduscin synthesis on bacterial virulence and phenoloxidase inhibition in a Spodoptera model. We first showed that the rhabduscin cluster of the entomopathogenic bacterium Xenorhabdus nematophila was not necessary for virulence in the larvae of Spodoptera littoralis and Spodoptera frugiperda . Bacteria with mutations affecting the rhabduscin synthesis cluster (Δ isnAB and Δ GT mutants) were as virulent as the wild-type strain. We then developed an assay for measuring phenoloxidase activity in S . frugiperda and assessed the ability of bacterial culture supernatants to inhibit the insect phenoloxidase. Our findings confirm that the X . nematophila rhabduscin cluster is required for the inhibition of S . frugiperda phenoloxidase activity. The X . nematophila Δ isnAB mutant was unable to inhibit phenoloxidase, whereas Δ GT mutants displayed intermediate levels of phenoloxidase inhibition relative to the wild-type strain. The culture supernatants of Escherichia coli and of two entomopathogenic bacteria, Serratia entomophila and Xenorhabdus poinarii , were unable to inhibit S . frugiperda phenoloxidase activity. Heterologous expression of the X . nematophila rhabduscin cluster in these three strains was sufficient to restore inhibition. Interestingly, we observed pseudogenization of the X . poinarii rhabduscin gene cluster via the insertion of a 120 bp element into the isnA promoter. The inhibition of phenoloxidase activity by X . poinarii culture supernatants was restored by expression of the X . poinarii rhabduscin cluster under the control of an inducible P tet promoter, consistent with recent pseudogenization. This study paves the way for advances in our understanding of the virulence of several entomopathogenic bacteria in non-model insects, such as the new invasive S . frugiperda species in Africa.

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Determinar la aptitud combinatoria de líneas endogámicas de maíz es de suma importancia para su desarrollo, por lo cual, en este estudio, se evaluaron 15 mestizos en tres localidades de Morelos, México (P-V 2017); 36 cruzas dialélicas (O-I 2018/2019) en una sola localidad, así como 15 líneas endogámicas per se (P-V 2018). Los objetivos de dichas evaluaciones fueron estimar y seleccionar líneas con buena aptitud combinatoria a través de prueba de mestizos; determinar si las líneas de alta Aptitud Combinatoria General (ACG) positiva para rendimiento de grano forman cruzas de alta Aptitud Combinatoria Específica (ACE) positiva con líneas de ACG negativa, y evaluar el potencial agronómico de líneas de diferente nivel de endogamia. Las variables analizadas fueron la floración masculina, altura de planta, peso de 100 semillas y rendimiento. Las líneas CML341, CML576 y CML549 resultaron con ACG positiva en mestizos y cruzas dialélicas, y las CML340, CML342, CML341, LUM124 y LUM194 fueron las de mayor rendimiento de grano.

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