The amb2 locus from Serratia entomophila confers anti-feeding effect on larvae of Costelytra zealandica (Coleoptera: Scarabaeidae)

Nuñez-Valdez, María Eugenia; Hk, Mahanty

doi:10.1016/0378-1119(96)00055-8

Cited by 10 publications

(12 citation statements)

References 6 publications

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“…DNA sequence analysis of the pAF6 clone revealed a region of high DNA sequence similarity and, accordingly, translated protein similarity to the gene products of the previously documented amb2 locus (39) (Table 3), a locus speculated to encode antifeeding activity against C. zealandica. Results from the present study and a previous study (22) indicated that the amb2 locus is not directly involved in the antifeeding process.…”

Section: Discussionmentioning

confidence: 75%

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

2004

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Serratia entomophila and Serratia proteamaculans (Enterobacteriaceae) cause amber disease in the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. Larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. A 155-kb plasmid, pADAP, carries the genes sepA, sepB, and sepC, which are essential for production of amber disease symptoms. Transposon insertions in any of the sep genes in pADAP abolish gut clearance but not cessation of feeding, indicating the presence of an antifeeding gene(s) elsewhere on pADAP. Based on deletion analysis of pADAP and subsequent sequence data, a 47-kb clone was constructed, which when placed in either an Escherichia coli or a Serratia background exerted strong antifeeding activity and often led to rapid death of the infected grass grub larvae. Sequence data show that the antifeeding component is part of a large gene cluster that may form a defective prophage and that six potential members of this prophage are present in Photorhabdus luminescens subsp. laumondii TTO1, a species which also has sep gene homologues.

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Section: Discussionmentioning

confidence: 75%

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

2004

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“…Another locus, amb2, that is required for induction of both symptoms of amber disease has already been described for S. entomophila (32). The cloned amb2 locus confers a cessationof-feeding effect on E. coli strains harboring it, and amb2 mutants of S. entomophila are nonpathogenic.…”

Section: Discussionmentioning

confidence: 99%

Plasmid-Located Pathogenicity Determinants of Serratia entomophila , the Causal Agent of Amber Disease of Grass Grub, Show Similarity to the Insecticidal Toxins of Photorhabdus luminescens

et al. 2000

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Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. Larval disease symptoms include cessation of feeding, clearance of the gut, amber coloration, and eventual death. A 115-kb plasmid, pADAP, identified in S. entomophila is required for disease causation and, when introduced into Escherichia coli, enables that organism to cause amber disease. A 23-kb fragment of pADAP that conferred disease-causing ability on E. coli and a pADAP-cured strain of S. entomophila was isolated. Using insertion mutagenesis, the pathogenicity determinants were mapped to a 17-kb region of the clone. Sequence analysis of the 17-kb region showed that the predicted products of three of the open reading frames (sepA, sepB, and sepC) showed significant sequence similarity to components of the insecticidal toxin produced by the bacterium Photorhabdus luminescens. Transposon insertions in sepA, sepB, or sepC completely abolished both gut clearance and cessation of feeding on the 23-kb clone; when recombined back into pADAP, they abolished gut clearance but not cessation of feeding. These results suggest that SepA, SepB, and SepC together are sufficient for amber disease causation by S. entomophila and that another locus also able to exert a cessation-of-feeding effect is encoded elsewhere on pADAP.

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“…Oral inoculation tests with bacteria were done basically as reported by Nuñez-Valdez and Mahanty (28) by feeding the larvae with small cylinders of fresh carrot (approximately 16 mm 3 each) coated with a single dose of 10 8 bacteria per larva. Control larvae were fed with uncoated pieces of carrot.…”

Section: Methodsmentioning

confidence: 99%

“…However, 72-h CCB administrated by injection caused mortality to P. blanchardi and to A. donovani larvae (Table 1), suggesting the presence of different toxic proteins acting at different levels in the process of pathogenicity: at the level of the insect gut, causing AFE as a first step, and also as a second step at the level of the hemocoel, causing mortality. Previous genetic evidence has suggested that toxin-like proteins were involved in amber disease caused by S. entomophila A1MO2 infection of C. zealandica (28,18,17). Analysis of the S. entomophila Mor4.1 and UC9 CCB by SDS-PAGE has shown the presence of a series of proteins ranging in estimated molecular mass from approximately 17 kDa to more than 97 kDa in both strains, which might be associated with AFE and mortality.…”

Section: Vol 74 2008 S Entomophila Strain Pathogenic To Scarabaeidmentioning

confidence: 97%

“…Sequencing data and genetic evidence have shown that the antifeeding component is part of a large gene cluster that forms a defective prophage (17) containing three DNA regions, (i) the amb2 locus, (ii) the intact lysis cassette, and (iii) the antifeeding prophage cluster. Several genes located in these regions (17,28) and upstream of the prophage cluster (18) have been implicated in the AFE. It has been proposed that the afp18 gene might encode an active protein responsible for antifeeding activity in the grass grub larvae (17).…”

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confidence: 99%

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Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

Nuñez-Valdez

Calderón

Aranda

et al. 2008

Appl Environ Microbiol

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The larvae of scarab beetles, known as "white grubs" and belonging to the genera Phyllophaga and Anomala (Coleoptera: Scarabaeidae), are regarded as soil-dwelling pests in Mexico. During a survey conducted to find pathogenic bacteria with the potential to control scarab larvae, a native Serratia sp. (strain Mor4.1) was isolated from a dead third-instar Phyllophaga blanchardi larva collected from a cornfield in Tres Marías, Morelos, Mexico. Oral bioassays using healthy P. blanchardi larvae fed with the Mor4.1 isolate showed that this strain was able to cause an antifeeding effect and a significant loss of weight. Mortality was observed for P. blanchardi, P. trichodes, and P. obsoleta in a multidose experiment. The Mor4.1 isolate also caused 100% mortality 24 h after intracoelomic inoculation of the larvae of P. blanchardi, P. ravida, Anomala donovani and the lepidopteran insect Manduca sexta. Oral and injection bioassays were performed with concentrated culture broths of the Mor4.1 isolate to search for disease symptoms and mortality caused by extracellular proteins. The results have shown that Mor4.1 broths produce significant antifeeding effects and mortality. Mor4.1 broths treated with proteinase K lost the ability to cause disease symptoms and mortality, in both the oral and the injection bioassays, suggesting the involvement of toxic proteins in the disease. The Mor4.1 isolate was identified as a putative Serratia entomophila Mor4.1 strain based on numerical taxonomy and phylogenetic analyses done with the 16S rRNA gene sequence. The potential of S. entomophila Mor4.1 and its toxins to be used in an integrated pest management program is discussed.

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The amb2 locus from Serratia entomophila confers anti-feeding effect on larvae of Costelytra zealandica (Coleoptera: Scarabaeidae)

Cited by 10 publications

References 6 publications

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

CloningSerratia entomophilaAntifeeding Genes—a Putative Defective Prophage Active against the Grass GrubCostelytra zealandica

Plasmid-Located Pathogenicity Determinants of Serratia entomophila , the Causal Agent of Amber Disease of Grass Grub, Show Similarity to the Insecticidal Toxins of Photorhabdus luminescens

Identification of a Putative Mexican Strain ofSerratia entomophilaPathogenic against Root-Damaging Larvae of Scarabaeidae (Coleoptera)

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