The lipopolysaccharide biosynthesis core of the Mexican pathogenic strain Serratia entomophila is associated with toxicity to larvae of Phyllophaga blanchardi

Rodríguez-Segura, Zitlhally; Chen, Jianwu; Villalobos, Francisco J.; Gill, Sarjeet S.; Nuñez-Valdez, María Eugenia

doi:10.1016/j.jip.2012.01.008

Cited by 10 publications

(4 citation statements)

References 37 publications

(48 reference statements)

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“…In addition to infection of human hosts, members of multiple Serratia species represent insect pathogens or are otherwise associated with insects. Serratia entomophila has been used as a biocontrol agent in New Zealand to predate upon the pasture pest, Costelytra zealandica 12,[22][23][24][25] , and S. proteamaculans 26 and S. marcescens 13,[27][28][29] have also been shown to be insect pathogens. In contrast, S. ficaria is associated with the pollination and oviposition cycle between figs and fig wasps, respectively 6 .…”

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The genus Serratia revisited by genomics

et al. 2022

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The genus Serratia has been studied for over a century and includes clinically-important and diverse environmental members. Despite this, there is a paucity of genomic information across the genus and a robust whole genome-based phylogenetic framework is lacking. Here, we have assembled and analysed a representative set of 664 genomes from across the genus, including 215 historic isolates originally used in defining the genus. Phylogenomic analysis of the genus reveals a clearly-defined population structure which displays deep divisions and aligns with ecological niche, as well as striking congruence between historical biochemical phenotyping data and contemporary genomics data. We highlight the genomic, phenotypic and plasmid diversity of Serratia, and provide evidence of different patterns of gene flow across the genus. Our work provides a framework for understanding the emergence of clinical and other lineages of Serratia.

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The genus Serratia revisited by genomics

et al. 2022

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“…The fact that the purified 50 kDa protein is insecticidal towards P. blanchardi supports this hypothesis. However, it is possible that other factors different to proteins might be implicated in the toxic activities of the bacteria, since it has been shown that bacterial wall components such as lipopolysaccharides might play an important role in pathogenicity to insects [ 15 ] and might be toxic to P. blanchardi larvae [ 16 ].…”

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confidence: 99%

Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)

et al. 2015

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Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology.

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“…Pest management strategies, which were discussed in this review, explain the application and design of microbial-derived management strategies in agriculture. A study on Serratia entomophila strain Mor4.1, a bacterial pathogen of several soil pests from genera: Phyllophaga and Anomala lead to the discovery of cell membrane protein having a toxic effect, which is proposed to be used in designing biocontrol project ( Rodríguez-Segura et al, 2012 ).…”

Section: Metagenomics and Sustainable Agriculturementioning

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Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review

et al. 2022

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Agricultural productivity is highly influenced by its associated microbial community. With advancements in omics technology, metagenomics is known to play a vital role in microbial world studies by unlocking the uncultured microbial populations present in the environment. Metagenomics is a diagnostic tool to target unique signature loci of plant and animal pathogens as well as beneficial microorganisms from samples. Here, we reviewed various aspects of metagenomics from experimental methods to techniques used for sequencing, as well as diversified computational resources, including databases and software tools. Exhaustive focus and study are conducted on the application of metagenomics in agriculture, deciphering various areas, including pathogen and plant disease identification, disease resistance breeding, plant pest control, weed management, abiotic stress management, post-harvest management, discoveries in agriculture, source of novel molecules/compounds, biosurfactants and natural product, identification of biosynthetic molecules, use in genetically modified crops, and antibiotic-resistant genes. Metagenomics-wide association studies study in agriculture on crop productivity rates, intercropping analysis, and agronomic field is analyzed. This article is the first of its comprehensive study and prospects from an agriculture perspective, focusing on a wider range of applications of metagenomics and its association studies.

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The lipopolysaccharide biosynthesis core of the Mexican pathogenic strain Serratia entomophila is associated with toxicity to larvae of Phyllophaga blanchardi

Cited by 10 publications

References 37 publications

The genus Serratia revisited by genomics

The genus Serratia revisited by genomics

Pathogenicity of Isolates of Serratia Marcescens towards Larvae of the Scarab Phyllophaga Blanchardi (Coleoptera)

Potential Use of Microbial Community Genomes in Various Dimensions of Agriculture Productivity and Its Management: A Review

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