Introduction: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia ("La Balsa"). Methods: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed. Results: Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/ American clade. Conclusions: This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics.
Bats are mammals of great ecological and medical importance, which have associations with different pathogenic microorganisms. DNA barcoding is a tool that can expedite species identification using short DNA sequences. In this study, we assess the DNA barcoding methodology in bats from the Colombian Northern region, specifically in the Córdoba department. Cytochrome oxidase subunit I (COI) gene sequences of nine bat species were typified, and their comparison with other Neotropic samples revealed that this marker is suitable for individual species identification, with ranges of intra-species variation from 0.1 to 0.9%. Bat species clusters are well supported and differentiated, showing average genetic distances ranging from 3% between Artibeus lituratus and Artibeus planirostris, up to 27% between Carollia castanea and Molossus molossus. C. castanea and Glossophaga soricina show geographical structuring in the Neotropic. The findings reported in this study confirm DNA barcoding usefulness for fast species identification of bats in the region.
Objective. Our aim was evaluate the presence of phlebotomine sandflies in an important rural area with eco-epidemiological interest in San Bernardo del Viento (Córdoba, Colombia). Materials and Methods. The insects identified were collected using CDC-traps and manual aspirator in closed trees between May to August 2015. The specimens were conserved and fragmented for clarification of internal structures and identification of morphological keys. Results. Three species of phlebotomine sand flies were identified: Lutzomyia gomezi (Nitzulescu 1931), Pintomyia rangeliana (Ortiz 1952) y Micropygomyia cayennensis (Floch & Abonnenc 1941). Conclusions. The presence of L. gomezi, main vector of cutaneous leishmaniasis in Colombia, is a risk factor for rural people from the zone.
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