“…Detection of DENV in adult female Aedes aegypti mosquitoes remains a challenge due to the low infection rate (typically about 0.1%) observed in adult female mosquitoes [6]. However, recent advancements in molecular virus detection techniques, particularly nucleic acid ampli cation tests such as RT-PCR and real-time RT-PCR assays, have enabled researchers to directly detect DENV RNA in eld-collected mosquitoes [3,4,5,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21]. The sensitivity, speci city and speed of nucleic acid ampli cation tests provide an alternative to the traditional method (i.e.…”