Two types of probiotics were used in piglets. One product is a mixed culture of viable Lactobacillus acidophilus, Enterococcus faecium e Bifidobacterium bifidum. The second product is composed of inactivated Lactobacillus acidophilus cells. The piglets received two weekly oral doses for 30 days while a control group did not receive probiotics. All piglets were euthanized at the 30th day of life and the mesenteric lymph nodes, the small intestine, and blood samples were collected. The tissue samples were studied by light microscopy and the blood serum was analyzed by ELISA method. The treatment with the probiotic with viable cells produced higher serum levels of IgA (P<0.05) and more IgA expressing cells were found in the mesenteric lymph nodes than observed in the inactivated cells treatment or control groups (P<0.05). Also, intestinal villi were longer, crypts were deeper (P<0.05) and fecal coliform count was lower than found in the inactivated product (P<0.05). These results suggest that viable probiotics are more efficient than inactivated probiotics to induce immunostimulation and intestinal modifications in piglets, thus improving their health and development.
JACINTO, L.M.B., RODRIGUES, M.A.M. e GUIMARÃES, E.C. Aspectos físico-químicos e microbiológicos de leite orgânico e leite convencional. PUBVET, Londrina, V. 8, N. 9, Ed. 258, Art. 1711, Maio, 2014 PUBVET, Publicações em Medicina Veterinária e Zootecnia. Aspectos físico-químicos e microbiológicos ResumoObjetivou-se nesse trabalho a comparação físico-química e microbiológica do leite produzido em manejo orgânico em relação ao oriundo de produção convencional. Foram colhidas 30 amostras de 10 animais das raças Gir (puras de origem) e Girolando (nos graus de sangue: 1/4, 1/2 e 5/8), com períodos de lactação variados (entre o 2º e o 5º de lactação) em cada uma das duas propriedades, a Fazenda Felicidade, destinada à produção de leite orgânico e a Fazenda Peroba, destinada à produção de leite convencional situadas no município de Ituiutaba em Minas Gerais, no período dos meses de Fevereiro, Março e Abril. Estas foram submetidas à contagem de mesófilos, à determinação do teor de gordura, extrato seco e proteína e à contagem de JACINTO, L.M.B., RODRIGUES, M.A.M. e GUIMARÃES, E.C. Aspectos físico-químicos e microbiológicos de leite orgânico e leite convencional. PUBVET, Londrina, V. 8, N. 9, Ed. 258, Art. 1711, Maio, 2014 células somáticas. Para a análise estatística, foi utilizado o teste t Student (p < 0,05) comparando as médias obtidas em cada uma das análises. Concluiu-se com tal estudo que o leite orgânico apresentou maiores teores de gordura, extrato seco e proteína, e não houve diferença na contagem de mesófilos e na contagem de células somáticas em relação ao leite convencional. Physical-chemical and microbiological aspects of the organic or usual milk AbstractThe main purpose of this research is the comparison of the milk produced in organic management towards the one that comes from usual production.Thirty samples, from 10 pure breed animals (Gyr), and Girolando (on blood levels: 1/4, 1/2 e 5/8), during different lactation periods (between the 2º and 5º), were collected at: Felicidade Farm, designated to the organical milk production, and Peroba Farm, designated to usual milk production, both in Ituiutaba -Minas Gerais, during the months of February, March and April.These samples were submitted to mesophiles counting; fat content, dry extract and protein determination, and to somatic cells count. To the statistical analysis the test t Student (p < 0,05) was used, comparing the averages acquired on each of the analyses. It was concluded that the organical milk showed higher level of fat content, dry extract and protein, and there was no difference on mesophiles counting, neither on somatic cells counting toward the usual milk. Introdução:Há uma tendência mundial pela busca por alimentos seguros que tragam maiores benefícios á saúde do ser humano, isto implica um conhecimento prévio da composição nutricional do alimento, sua vida útil na prateleira, isenção de contaminantes, como defensivos químicos, hormônios, antibióticos JACINTO, L.M.B., RODRIGUES, M.A.M. e GUIMARÃES, E.C. Aspectos físico-químicos e microbi...
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