Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that λD is not the main factor governing the effective nanoscale screening environment. We observed that the longer λD was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity.
Combined microscopy techniques offer the life science research community a powerful tool to investigate complex biological systems and their interactions. Here, we present a new combined microscopy platform based on fluorescence optical sectioning microscopy through aperture correlation microscopy with a Differential Spinning Disk (DSD) and nanomechanical mapping with an Atomic Force Microscope (AFM). The illumination scheme of the DSD microscope unit, contrary to standard single or multi-point confocal microscopes, provides a time-independent illumination of the AFM cantilever. This enables a distortion-free simultaneous operation of fluorescence optical sectioning microscopy and atomic force microscopy with standard probes. In this context, we discuss sample heating due to AFM cantilever illumination with fluorescence excitation light. Integration of a DSD fluorescence optical sectioning unit with an AFM platform requires mitigation of mechanical noise transfer of the spinning disk. We identify and present two solutions to almost annul this noise in the AFM measurement process. The new combined microscopy platform is applied to the characterization of a DOPC/DOPS (4:1) lipid structures labelled with a lipophilic cationic indocarbocyanine dye deposited on a mica substrate.
MEMS fabricated on polymer substrates can allow for a range of new applications that require bending or lightweight, unbreakable substrates. The surface micromachining of hydrogenated amorphous silicon resonators on 10 µm-thick flexible polyimide substrates is presented. Clamped-clamped (bridges) and clamped-free (cantilevers) resonators are fabricated and characterized, exhibiting quality factors as high as 2.0 × 103 and natural resonance frequencies in the 104–106 Hz range. The deflection of an 80 µm long bridge was measured to be over 100 pm, using laser Doppler vibrometry. The electrical addressing of the devices was demonstrated to be reliable when bent to radii of curvature larger than 10 mm. The resonators on ultra-thin polymer are characterized using different actuation voltages and pressures, showing comparable performance to resonators on rigid (glass) substrates. However, the flexible substrate allows the relaxation of the residual stress of the structural film in clamped-clamped structures, lowering the resonance frequency. Resonators on PI were found to be suitable for mass-sensing applications, achieving a minimum frequency shift detectable Δfmin = 30 Hz which results in a calculated mass sensitivity of 25 pg in vacuum. Ultimately, the reliable performance of the resonators developed in this work make them good candidates for applications that require mass-sensing on ultra-thin, flexible substrates.
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