Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.

High-performance thin-layer chromatographic analysis with automated multiple development (AMD-HPTLC) of some commercial preparations and of laboratory-prepared extracts of Echinacea species is reported. Using an alkaline-based gradient system, hydrophilic and lipophilic compounds were separated in one chromatographic run. The chromatographic retention data were combined with spectroscopic data, i.e., UV and fluorescence spectra for the native compounds and after post-chromatographic derivatisation. Several isobutyramides and caffeic acid derivatives were identified. AMD-HPTLC fingerprint patterns were obtained for characterising and evaluating complex plant extracts.

This accessible guide presents the astrophysical concepts behind astronomical spectroscopy, covering both the theory and the practical elements of recording, processing, analysing and interpreting your spectra. It covers astronomical objects, such as stars, planets, nebulae, novae, supernovae, and events such as eclipses and comet passages. Suitable for anyone with only a little background knowledge and access to amateur-level equipment, the guide's many illustrations, sketches and figures will help you understand and practise this scientifically important and growing field of amateur astronomy, up to the level of Pro-Am collaborations. Accessible to non-academics, it benefits many groups from novices and learners in astronomy clubs, to advanced students and teachers of astrophysics. This volume is the perfect companion to the Spectral Atlas for Amateur Astronomers, which provides detailed commented spectral profiles of more than 100 astronomical objects.

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