Planar chromatography of Echinacea species extracts with automated multiple development

Abstract

High-performance thin-layer chromatographic analysis with automated multiple development (AMD-HPTLC) of some commercial preparations and of laboratory-prepared extracts of Echinacea species is reported. Using an alkaline-based gradient system, hydrophilic and lipophilic compounds were separated in one chromatographic run. The chromatographic retention data were combined with spectroscopic data, i.e., UV and fluorescence spectra for the native compounds and after post-chromatographic derivatisation. Several isobutyramides and caffeic acid derivatives were identified. AMD-HPTLC fingerprint patterns were obtained for characterising and evaluating complex plant extracts.

Qualitätssicherung pflanzlicher Arzneimittel. 4. Symposium der Fachgruppe Arzneimittelkontrolle/Pharmazeutische Analytik der Deutschen Pharmazeutischen Gesellschaft

“…Cichoriensaure ist allerdings relativ instabil und baut sich haufig bereits bei der Herstellung der Tinktur ah [16]. Fur die Fingerprintanalyse von entsprechenden Echinacea-Tinkturen wurde auch ein AMD-HPTLC-Verfahren (HPTLC mit automatischer Mehrfachentwicklung) unter Verwendung von Kieselgelplatten beschrieben [17] Fur die seit dem vorigen Jahrhundert beschriebene ,,Schafgarbenallergie [I], eine Kontaktallergie wurde als verannvortliche Substanz das Endoperoxyguaianolid a-Peroxyachifolid (I) isoliert [2]. Zur quantitativen Bestimmung dieser Verbindung in Pflanzenmaterial wurde ein densitometrisches Verfahren auf HPTLC-Platten eingesetzt [3].…”

Quantification of Phenylpropanoids in Commercial Echinacea Products Using TLC with Video Densitometry as Detection Technique and ANN for Data Modelling

“…Several studies have demonstrated the application of TLC for the qualitative analysis of Echinacea (Bauer et al ., ; Bauer and Remiger, ; Trypsteen et al ., ; Gocan et al ., ; Reich et al ., ) and only few studies have used TLC and slit‐scanning densitometry detection for the quantification of marker compounds in Echinacea (Georgian, ; Gilroy et al ., ; Tie‐xin and Hong, ).…”

Characterization of Orthosiphon Stamineus Benth extracts by reversed-phase thin layer Chromatographic methods

“…The AMD technique uses a solvent gradient and several development steps to separate compounds of widely different polarity. Most reported AMD applications have used gradients on normal phase plates [10][11][12][13][14][15][16][17][18][19]. This paper presents the qualitative and quantitative determination of rosmarinic acid, sinesetine and eupatorine from two extracts of Orthosiphon stamineus Benth.…”