Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea

Abstract

Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.

Induction of hairy roots in Arnica montana L. by Agrobacterium rhizogenes

“…Moreover, they used a different strain A. rhizogenes LBA 9402. The higher transformation frequency was achieved in other members of the Asteraceae family, using different explants (hypocotyls, cotyledons, leaves): Artemisia annua (10-100%) [36], Echinacea purpurea (40%) [37], Cichorium inthybus (30%) [38], Taraxacum platycarpum (16.2 to 76.5%) [39]. The induced hairy roots showed different morphology and growth patterns on MS nutrient media.…”

Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare

“…A. rhizogenes strains ATCC15834 and K599 belong to the agropine-type Ri-plasmids, which are very similar as a group (Sevón et al 2002). Trypsteen et al (1991) showed that the overall process of T-DNA integration in an agropine type is very similar (Trypsteen et al 1991). Tinland (1996) showed that the age and differentiation status of plant tissue and the level of tissue differentiation can affect hairy root induction after inoculation with A. rhizogenes (Tinland 1996).…”

In vitro production of thiophenes using hairy root cultures of Tagetes erecta (L.)

“…They were surface sterilized with 0.1% mercuric chloride for 90 s, and washed three times with autoclaved distilled water for 10 min each. The disinfected seeds were germinated on media containing MS salts (Murashige and Skoog, 1962) (Trypsteen, 1991). The young leaves obtained from in vitro grown T. erecta plants were used as explants source.…”