The administration of hormone GnRH will affect the reproductive organs and can improve the reproductive performance of buffalo. The decrease of the buffalo population is caused by various factors including genetic and environmental factors. The development of buffaloes is relatively slow because the reproduction rate of buffaloes is lower than that of cattle, besides that it is also difficult to detect estrus, the pregnancy period is relatively long and longer calving rate. This study aims to determine the best dose of GnRH on the swamp buffalo producing of dadih in Indonesia. The materials used were 16 swamp buffalo. A completely randomized design was used in this study. Buffalo is synchronized with the injection of 5 ml of GnRH hormone n on the first and 11th day, on the 10th to 12th day all donor buffaloes are injected with GnRH. Four different doses of GnRH (200, 225, 250 and 275 ml/buffalo) and each treatment was replicated five times. The speed of estrus and estrus length were the measured variables. The results showed that the doses of GnRH significantly (P<0.05) increase of estrus speed and estrus length. Progesterone levels have increased significantly starting from before treatment until after treatment with GnRH. It is concluded that the best GnRH doses for estrus synchronization in the swamp buffalo producing of dadih was 2. 25 ml/buffalo with estrus speed 18.2 h and estrus length 18 h.
This study aimed to resequencing of 962 bp of exon 9 and intron 9 of follicle stimulating hormone receptor (FSHR) gene in Pesisir cattle to identify single nucleotide polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in the exon 9 and intron 9 regions, which were located at the 18 A>G, 213 T>C, +49 A>T, +232 A>C, and +252 G>A. There were 3 transition type mutations (at positions 8, 213 and +252) and 2 transversion type mutations (at positions +49 and +232). Based on the result of this study, it can be stated that the genotype frequency of Pesisir cattle population was not in the Hardy-Weinberg equilibrium.
This study was conducted to determine the population performance of the kacang goats as natural resources in Talawi District, Sawahlunto Regency. This study was carried out on 628 small farmers in Talawi district in Sawahlunto for approximately two months, from June to August 2022. The selection of the research location was through purposive sampling. In collecting the data, the researchers applied direct observation (survey), interviews with the farmer using a questionnaire, and documentation. The data was collected form of primary and secondary data. In this study, the research variables were the population of the kacang goat, livestock input, livestock output, and natural increase value. The data in this study were tabulated and analyzed using descriptive statistical analysis. The results showed that the highest Kacang goats in Talawi District population were adult females, with as 281 heads or 44.74% of the total population. The lowest population was found in the young male, 39 heads or 6.21% of the total population. Moreover, livestock input was 29.14% from births and 6.37% from purchases. Furthermore, the output of kacang goats was 22.09% death, 2.71% slaughter, 9.07% sales, and natural increase. It can be concluded that the population performance of the Kacang goat was not in good condition. Therefore, improving the reproductive performance of adult female goats and the rearing system of newborns until the weaning of goats needs to be done in this area.
The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.
This study aimed to determine the use of various doses of FSH hormone, the number of lutheum corpus and the number and quality of embryos produced in the superovulation program in Pesisir cattle. The experimental design was Analysis of Variance (Anova) in a completely randomized design. The research material used 16 cows from Pesisir cattle that had been synchronized with estrous with CIDR implants for 13 days and were injected with the PGF2α hormone after the release of CIDR, then scheduled AI was performed. The dose of FSH hormone was 16 ml, 17 ml, 18 ml and 19 ml, each treatment consisting of 4 replications. The injection of the FSH hormone was carried out for 3 days at a decreased dose. Collection of donor embryos was carried out on days 6 to 8 after the scheduled AI. The parameters observed included superovulation response, number of corpus luteum, number of embryos and quality of embryos. The results showed that the response of Pesisir cattle superovulation was 81.25%. The average number of CL obtained was 13.75 and embryos for Pesisir cattle was 11.25. The results also showed that the quality of the embryos obtained were grade A 19, grade B 18, grade C 7, grade D 1. It can be concluded that the use of various doses of FSH hormone at doses of 16 mg, 17 ml, 18 ml and 19 ml had shown a superovulatory response. The 17 ml dose of FSH hormone showed a large number of embryos and had the best embryo quality.
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