The Effect of GnRH on Reproductive Performance and Progesterone Hormone Levels in Buffalo in West Sumatera

Abstract

The administration of hormone GnRH will affect the reproductive organs and can improve the reproductive performance of buffalo. The decrease of the buffalo population is caused by various factors including genetic and environmental factors. The development of buffaloes is relatively slow because the reproduction rate of buffaloes is lower than that of cattle, besides that it is also difficult to detect estrus, the pregnancy period is relatively long and longer calving rate. This study aims to determine the best dose of GnRH on the swamp buffalo producing of dadih in Indonesia. The materials used were 16 swamp buffalo. A completely randomized design was used in this study. Buffalo is synchronized with the injection of 5 ml of GnRH hormone n on the first and 11th day, on the 10th to 12th day all donor buffaloes are injected with GnRH. Four different doses of GnRH (200, 225, 250 and 275 ml/buffalo) and each treatment was replicated five times. The speed of estrus and estrus length were the measured variables. The results showed that the doses of GnRH significantly (P<0.05) increase of estrus speed and estrus length. Progesterone levels have increased significantly starting from before treatment until after treatment with GnRH. It is concluded that the best GnRH doses for estrus synchronization in the swamp buffalo producing of dadih was 2. 25 ml/buffalo with estrus speed 18.2 h and estrus length 18 h.

Addition of Trehalose of Duck Egg Yolk-Tris as an Extender Medium on Buffalo Frozen Semen

“…Based on the results of the average percentage of motility in buffalo in each ejaculation with different concentrations of trehalose, it can be seen that the average motility of frozen semen is the highest 20.00±0.00% at the control concentration and the lowest ie 15.00±5.77% . This is in accordance with the opinion of Arifiantini et al [19] who stated that the motility of spermatozoa post thawing is at least 40%, if less than 40%, the frozen semen is not suitable for insemination [3]. The difference in the percentage of motility of buffalo spermatozoa after freezing is caused by the level of trehalose concentration used as a treatment.…”

“…Low semen quality was one of the causes of AI failure. Related to this problem, various strategies to improve the quality of semen have been carried out, especially through improving the freezing process or cryopreservation, so that frozen semen can be used for a long time [2,3]. Besides, the quality of the semen produced can affect the success of AI, right extender methods and storage of semen are also known to be able to influence the success of AI.…”