Genus Mucuna which is native to China and Eastern India comprises of perennial climbing legume with long slender branches, trifoliate leaves and bear green or brown pod covered with soft or rigid hairs that cause intense irritation. The plants of this genus are agronomically and economically important and commercially cultivated in India, China and other regions of the world. The high degrees of taxonomical confusions exist in Mucuna species that make authentic identification and classification difficult. In the present study, the genetic diversity among the 59 accessions of six species and three varieties of M. pruriens has been assessed using DNA fingerprinting based molecular markers techniques namely randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and combined dataset of RAPD and ISSR. Also, genetic relationship among two endemic species of Mucuna namely M. imbricata and M. macrocarpa and two varieties namely IIHR hybrid (MHR) and Dhanwantari (MD) with other species under study was investigated by using cluster analysis and principal coordinate analysis.The cluster analysis of RAPD, ISSR and combined dataset of RAPD and ISSR clearly demonstrated the existence of high interspecific variation than intra-specific variation in genus Mucuna. The utility and efficacy of RAPD and ISSR for the study of intra species and interspecies genetic diversity was evident from AMOVA and PCoA analysis. This study demonstrates the genetic diversity in Mucuna species and indicates that these markers could be successfully used to assess genetic variation among the accessions of Mucuna species.
In order to effectively purify drinking water or wastewater, harmful organic contaminants like nitroarenes must be removed from water systems. In this context, the present study developed a unique bio-mimetic, in-situ synthesis of Ag/rGO nanocomposite. We employed Bos taurus indicus urine to make Ag/rGO nanocomposite in an environmentally friendly, economical, and sustainable manner. Through the use of DLS, FE-SEM, FT-IR, Raman, TGA-DSC, XRD, and Zeta Potential analysis, the morphological, elemental, and structural characterization were completed. Investigations were conducted into the catalytic effectiveness of prepared nanomaterials for diverse nitroarenes reduction. Then, using NaBH4 at 25oC, the catalytic roles of Ag and Ag/rGO nanocatalyst were assessed towards the catalytic reduction of several environmental pollutants such as 2,3 & 4-nitroaniline and 4-nitrophenol. For their catalytic performance, bio-mimetically synthesized Ag NPs were thermally treated at 200°C and compared to Ag/rGO nanocomposite. The antibacterial and antioxidant properties of as-prepared nanomaterials were investigated in this study. The finding signifies the importance of such nanocomposites in medicinal chemistry and antibacterial creams for external use.
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