Job rotation is a type of on-the-job knowledge acquiring where an employee is encouraged through a list of coursework planned to give them an extent of practical knowledge and coverage of different aspects of employment for that profession. Job rotation is a suitable development method for employees who are focusing only on common job tasks rather than a specialized professional route. It is also a way of obtaining speedy coverage and experience to a large selection of positions within a company in order to boost certain capabilities. The basic purpose of this study is to find out the impact of job rotation on employees in UAE public and private (both) organizations. For this purpose, online survey technique was used and data was gathered from 100 employees including males and females from UAE organizations. Data was analyzed through reliability, descriptive, correlation and regression analysis. The findings of the study show that job rotation has a highly significant and positive impact on employees in both the public and private sector in United Arab Emirates organizations. Job rotation creates new opportunities for employees and develops high skills in them.