Aims:To investigate the effect of ethanolic extract of Salvadora Persica extract on HSV-1 infection both in vitro and in vivo in the mouse model system. Materials and methods: Ethanolic extract of Salvadora Persica was used at different concentrations. BHK cells that grown in Eagles medium were used for virus isolation and titration using PFU/ml. The effects of different concentrations of Salvadora Persica on viral growth in BHK cells as well as cytolytic activity of HSV-1 were evaluated at different time post infection. The therapeutic efficacy of Salvadora Persica in vivo was studied in mice. Lesions were scored and viral isolation from infected skin and ganglia was titrated on BHK cells. Results: Salvadora Persica inhibited the replication of HSV-1 in BHK cells as well as the cytolytic activity of cell free virus. Topical application of Salvadora Persica on the skin of mice infected with HSV-1 reduced the development of cutaneous lesions and the viral titers in the skin and ganglia were also reduced. Conclusion: The results of this work may be beneficial for the treatment of recurrent oral herpes infections.
Aim: To compare the effectiveness of four different disinfectant solutions in rapid decontamination of gutta percha cones. Materials and Methods: In this experimental study, 320 gutta percha cones were placed in bacterial suspensions of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Candida albicans for 30 minutes, and then immersed in disinfectant solutions (6% hydrogen peroxide, 2% chlorhexidine, 1% sodium hypochlorite, and 70% ethyl alcohol) for 1, 5, 10, and 15 minutes. After that, the cones were aseptically transferred to the test tubes containing 4 ml brain heart infusion broth, and then cultured on selected agar media and number of colonies was estimated after 24 hours incubation at 37 o C. Results: Hydrogen peroxide was effective in 1 minute, while chlorhexidine and sodium hypochlorite after 10 minutes, and ethyl alcohol in 5 minutes for decontamination of gutta percha cones. Conclusion: Hydrogen peroxide was the most effective agents in decontamination of gutta perch cones.
Aims: The aim of the study is to assess the blood levels of (interleukin-6) at different intervals of time after surgical removal of impacted lower third molars.. Materials and Methods: The study group comprised 26 patients with impacted lower third molars .All extractions were performed under local anaesthesia using the inferior alveolar nerve block together with vestibular infiltration. A buccal full thickness two sided mucoperiosteal flap was raised. Sterile low-speed straight hand pieces and sterile distilled water were used for ostectomy. After tooth removal the wound was closed with 3-0 black silk suture. Three blood samples were drawn from each patient; one before the operation, the second 2hr. post operation and the third 24hr. post operation. The blood samples were collected through venipuncture, serum separated and stored in at-20ºC. Analysis was performed using the Enzyme linked Immunosourbant assay (ELISA). Results: The level of IL-6, in this study, showed an increase following surgery, in comparison with the level before operation. This increase in the level of Interleukin-6 remained high until 24hr after operation. Conclusion: Interleukin-6 (IL-6) is higher after surgical extraction of lower third molars and remained with high levels to 24hr. after operation.
Aims:To evaluate the antimicrobial activity of MTA against selected microorganisms compared with a widely used root end filling materials. Materials and methods: Fifteen mm discs of MTA, GIC and Amalgam were prepared and three types of microorganisms; two bacteria and one fungus, were grown in 4 ml of brain heart infusion broth for 18 hr. Then 0.5 ml of each growth was spread over selected media (three plates for each sample) and the discs were applied on the agar, incubated for 24-48 hr and the zone of inhibition was measured. Results: Amalgam did not demonstrate any antimicrobial activity, whereas MTA showed antimicrobial effect against all tested microorganisms and was highly significant. GIC showed antibacterial activity comparable to MTA but failed to produce antifungal effect. Conclusions: MTA demonstrated antibacterial and antifungal effect, while GIC showed only antibacterial activity, whereas Amalgam did not show any activity.
Aims: To identify the clinical picture and the association between dry socket and severe postoperative development with one of the systemic risk factors (typhoid fever). Materials and Methods: Twenty adult (both sexes) patients attended dental clinic searching for different dental treatments were studied. The clinical picture of 6 months period study was evaluated by completing two case sheet forms. They were diagnosed having dry sockets and severe postoperative pain. Blood samples were taken for bacteriological, serological and hematological study to diagnose typhoid fever. These tests included WAT, WFT, WBCs and blood culture. Results: Significant correlation existed between postoperative pain and typhoid fever examined by BC and the highest percentage was with dry socket (61.5%) followed by WFT (57.1%(, WAT (44.4%) using two tests for diagnosis showed that WFT and BC gave 97.9% positive for dry socket and Kappa test was 88%, while WAT and WBCs or WFT and WAT gave the least non significant correlation. Conclusion: There was a strong correlation between dry socket and typhoid fever and the most sensitive tests for diagnosis was BC followed by WFT, WBCs and WAT.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.