This study aims to determine the chemical composition and evaluate the antibacterial activity of the essential oil of leaves dry lavender (Lavandula officinalis). The analysis of the essential oil indicates the presence of terpenes, tannins, saponins, flavonoids, alkaloid, coumarins, and the absence of anthocyanins. Furthermore, of the oil's antimicrobial activity was evaluated using agar diffusion and broth microdilution methods. The antimicrobial test results showed that the oil had antimicrobial activity against all 8 microorganisms strains included in the study. Results suggest antimicrobial properties of the Lavandula officinalis essential oil, which may find its application in future research for the pharmaceutical industry.
Salmonella enterica serovar typhimurium is the first cause identified of food poisoning in humans; new data indicate that the resistant strains of Salmonella sp. can be inhibited by a bacterial population present in foods have been called probiotics. Our study was conducted on Swiss albino mice in order to know the effect of probiotics or biotherapeutic agents, and suggest the different therapeutic possibilities of intestinal complications related to the presence of Salmonella enterica serovar typhimurium; Reference strains, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus and Lactobacillus paracasei in combination with Bifidobacterium sp. were tested for their health benefits. The number of Salmonella enterica serovar typhimurium decreases in intestine, in the stomach and in the liver for group 2, 3 in case of preventive treatment and group 4, 5 in case of a therapeutic treatment due to the use of a culture of probiotics, in comparison with group 1 where we recorded a high number. The bio-therapeutic treatment was finally confirmed by macroscopic and microscopic observations of different target organs before and after treatment for the experiment period.
Phenol is the major organic pollutant present in industrial waste water. Phe-nol biodegradation is the most efficient and cost-effective pollutants degra-dation technologies, it can also ensure a completely degradation of phenol on non toxic products .In this research our objective is to isolate a new bac-terial strains with the ability of degrading phenol at high concentrations. Four phenol-degrading bacterium strain were successfully isolated from the effluent of industrial wastewater collected from Telamine Lake in the indus-trial area in Oran. Based on their morphological, physiological and biochemi-cal characteristics, all the strains were characterized as a Gram-negative, strictly aerobic, no motile and short rod-shaped bacterium that utilize phe-nol as a sole carbon and energy source. 16S rRNA sequence analysis revealed that these strains ENP, ENP2, ENP3 and ENP4 were affiliated to Pseudomo-nas putida, Pseudomonas sp, Raoultella ornithinolytica and Klebisella Sp re-spectively in the group of Gammaproteo bacteria. All the strains were effi-cient in removing 99% of the initial 750 mg/L of phenol within 72 h and had a tolerance of phenol concentration as high as 1000 mg/L of phenol. Analysis of the gene of the largest subunit of the multi-component phenol hydrox-ylase (LmPH) in each isolate showed the presence of this one in all isolate and only the strains ENP1 and ENP2 had the Catechol 2,3-dioxygenases gene (C230). These results indicated that the strains ENP1, ENP2, ENP3 and ENP4 can be exploited for bioremediation of phenol.
This study was devoted to phytochemical screening and evaluation of biological properties, namely, the antimicrobial activities of hydroethanolic and infusion extracts prepared from Algerian Sahara Matricaria pubescens (Desf). Phytochemical tests applied to the studied plant showed the presence of several families of chemical compounds, including tannins and flavonoids. The in vitro antimicrobial activity of M. pubescens extracts was moderate, while the gram-positive bacteria were more sensitive than the gram-negative strains (MIC values between 10 and 20 mg/mL). The contemporary presence of bioactivities suggests that the Saharan M. pubescens (Desf) may be a source for such new preservatives in the food and pharmaceutical industries.
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