A novel Niallia nealsonii Bacteria Degrading Phenol Isolated From Oum Ghellaz Lake Shore Soil in Northern Algeria

Djamila, Maghnia; Fatma, Adoudj; abdesslam, Ait Abdessalem; Linda, Medouakh; Abdelkader, Ammam; Reda, Belmamoun

doi:10.21608/eajbsc.2022.289666

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These plates were then incubated at 20 °C, and single colonies with morphological differences were selected and streaked on new plates. This process allowed for the isolation and selection of different types of phenol-degrading bacteria [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

Design of a two functional permeable reactive barrier for synergistic enzymatic and microbial bioremediation of phenol-contaminated waters: laboratory column evaluation

Mirdamadian,

Asad,

Dastgheib

et al. 2024

BMC Microbiol

View full text Add to dashboard Cite

The present study aimed to develop a system using a combination of enzymatic and microbial degradation techniques for removing phenol from contaminated water. In our prior research, the HRP enzyme extracted from horseradish roots was utilized within a core-shell microcapsule to reduce phenolic shock, serving as a monolayer column. To complete the phenol removal process, a second column containing degrading microorganisms was added to the last column in this research. Phenol-degrading bacteria were isolated from different microbial sources on a phenolic base medium. Additionally, encapsulated calcium peroxide nanoparticles were used to provide dissolved oxygen for the microbial population. Results showed that the both isolated strains, WC1 and CC1, were able to completely remove phenol from the contaminated influent water the range within 5 to 7 days, respectively. Molecular identification showed 99.8% similarity for WC1 isolate to Stenotrophomonas rizophila strain e-p10 and 99.9% similarity for CC1 isolate to Bacillus cereus strain IAM 12,605. The results also indicated that columns using activated sludge as a microbial source had the highest removal rate, with the microbial biofilm completely removing 100% of the 100 mg/L phenol concentration in contaminated influent water after 40 days. Finally, the concurrent use of core-shell microcapsules containing enzymes and capsules containing Stenotrophomonas sp. WC1 strain in two continuous column reactors was able to completely remove phenol from polluted water with a concentration of 500 mg/L for a period of 20 days. The results suggest that a combination of enzymatic and microbial degrading systems can be used as a new system to remove phenol from polluted streams with higher concentrations of phenol by eliminating the shock of phenol on the microbial population.

show abstract

Section: Methodsmentioning

confidence: 99%