The increase in human population and domestic pets, such as cats, are generating important consequences in terms of habitat loss and pathogen pollution of coastal ecosystems with potential to generate negative impacts in marine biodiversity. Toxoplasma gondii is the etiological agent of zoonotic disease toxoplasmosis, and is associated with cat abundance and anthropogenic disturbance. The presence of T. gondii oocysts in the ocean has negatively affected the health status of the threatened Southern sea otter (Enhydra lutris nereis) populations. The present study analyzed seroprevalence and presence of T. gondii DNA in American mink (Neovison vison), Southern river otters (Lontra provocax) and domestic cats (Felis silvestris catus) in four different areas in Southern Chile comprising studies in rivers and lakes in Andean foothills and mountains, marine habitat and island coastal ecosystems. Mean seroprevalence of T. gondii in the study was 64% of 151 total animals sampled: 59% of 73 American mink, 77% of 13 Southern river otters, 68% of 65 domestic cats and in two of two kodkods (Leopardus guigna). Toxoplasma gondii DNA was detected in tissues from one American mink and one Southern river otter. The present study confirms the widespread distribution of T. gondii in Southern Chile, and shows a high exposure of semiaquatic mustelids and domestic cats to the parasite. Cats and anthropogenic disturbance have a role in the maintenance of T. gondii infection in ecosystems of southern Chile.

Background: Introduced species can have a major negative impact on biodiversity; an example is the American mink, which was introduced in the 1930s in Patagonia. While there is a consensus that reversing alien mustelids continental scale invasions remains unfeasible, there is little consensus, given a maximum cost or investment, on the feasibility and efficiency of region-wide control or eradication. Thus, our goal was to provide information about efficiency for mink control in Patagonia. Methods: Between January 2009 and February 2013 this study was conducted in ten study sites (4 km to 15 km long) between 39°S to 45°S latitude. Minks were trapped using cage traps operated by two trappers. We estimated the population density at each study site assuming they were close populations, exhibit intrasexual territoriality and the home range of females were smaller than those of males. We obtained a theoretical population and a modeled population from our trapping results. Sixty five minks were trapped over 2190 trap nights (0.03 mink/trap night). Mink captures were higher in the first six days and in the first trapping campaigns. A two person team was able to control a maximum distance of approximately 6 km of river shore by foot and 15 km of sea and lake shores by boat. There was an over linear increase of operational costs as time passed. Our modeled population was 91% of the theoretical population. Conclusions: We believe that to trap and remove a minimum of 70% of the mink population in a region under ideal circumstances, traps should be deployed every 200 m and after the sixth day should be moved to another new transect. We suggest an annual repetition of this strategy as the more efficient for controlling mink populations in terms of trapping success and reduced costs. The number of traps will depend on the number of trappers participating and also on habitat characteristics.

Objective: The present study aimed to vocally assess a group of rock singers who use growl voice and reinforced falsetto. Method: A group of 21 rock singers and a control group of 18 pop singers were included. Singing and speaking voice was assessed through acoustic, perceptual, functional and laryngoscopic analysis. Results: No significant differences were observed between groups in most of the analyses. Acoustic and perceptual analysis of the experimental group demonstrated normality of speaking voice. Endoscopic evaluation showed that most rock singers presented during singing voice a high vertical laryngeal position, pharyngeal compression and laryngeal supraglottic compression. Supraglottic activity during speaking voice tasks was also observed. However, overall vocal fold integrity was demonstrated in most of the participants. Slightly abnormal observations were demonstrated in few of them. Singing voice handicap index revealed that the most affected variable was the physical sphere, followed by the social and emotional spheres. Conclusions: Although growl voice and reinforced falsetto represent laryngeal and pharyngeal hyperfunctional activity, they did not seem to contribute to the presence of any major vocal fold disorder in our subjects. Nevertheless, we cannot rule out the possibility that more evident vocal fold disorders could be found in singers who use these techniques more often and during a longer period of time.

The interaction between native and introduced predators can be an important determinant of the success of introduced species and of the magnitude of their effects. In Europe, it has been shown that the American mink Neovison vison can be affected by native competitors; however, such evidence has not been found in South America. We studied the southern river otter Lontra provocax and the American mink at five marine sites, where they coexisted, and at one freshwater site, where only mink were present, in southern Chile. We used the signs of both species to study their habitat use and diet, and radio tracking to study their activity patterns. The results indicated that otters and minks tended to use different habitats in marine environments, the otter favouring littoral areas that are rocky and steep while the mink favours areas of gravel with a gently sloping intertidal zone. These differences were also reflected in their diets. At one of the coastal sites where the diet of the two species was similar, the activity pattern of minks was mostly diurnal, which is unusual. While differential habitat use may be the way through which the American mink is able to coexist with the southern river otter in coastal habitats of southern Chile, it is possible that otters are having an effect on individual minks by affecting their activity patterns, although more observations are needed to confirm this hypothesis.

Background: Leptospirosis is an important zoonosis with worldwide distribution caused by pathogenic bacteria of the genus Leptospira. The North American mink (Neovison vison) has an important role in the environmental contamination with Leptospira, as minks live in aquatic environments and are the predators of rodents. Findings: Blood and kidney samples were obtained from 57 minks in Southern Chile 39°S to 45°S. Pathogenic species of Leptospira were detected by PCR on 31/57 minks. To determine the species, we sequenced the 16S ribosomal RNA (rRNA) gene on nine of the positive samples. We predicted two pathogenic species: Leptospira interrogans (five samples) and Leptospira borgpetersenii (four samples).Conclusions: This study showed that the American mink presents pathogenic species of Leptospira and confirm important environmental contamination of Patagonian rivers and lakes with pathogenic Leptospira.

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