This study describes the preparation of nanoparticles derived from bovine serum albumin (BSA) in comparison with the formation of nanoparticles composed of human serum albumin (HSA), when the same preparation procedure was used in both cases. To obtain protein nanoparticles, the method of desolvation with ethanol was employed, followed by the stabilization with urea and cysteine. It was shown that, upon transition from HSA to BSA, the particles with smaller sizes and with a narrower polydispersity were formed. The possibility of the immobilization of the antitumor drug hydroxyurea in such protein nanoparticles by adsorption and inclusion methods has been shown. The drug release profile from the polymer matrix was established.
Background. The research results of fat-soluble vitamin D3 (cholecalciferol) encapsulation with β-cyclodextrin have been presented in this work. The vitamin D3 inclusion complex with β-cyclodextrin was obtained under microwave radiation. The surface morphology of obtained clathrate inclusion complexes was described with the help of a scanning electron microscope. The thermographic measurement results on a differential scanning calorimeter have been presented. The activation energy of the β-cyclodextrin : vitamin D3 clathrate complex thermal oxidative destruction reaction was calculated. The clathrate thermal destruction kinetic parameters were determined. The inclusion complex spectral properties were characterized by IR-Fourier and 1H and 13C NMR spectroscopy. The existence of β-cyclodextrin inclusion complex with vitamin D3 in a 2 : 1 ratio was confirmed by the experimental results. The activation energy of thermal destruction of the inclusion complex of β-cyclodextrin with vitamin D3 was calculated using four different methods.
Tuberculosis is one of the dangerous infectious diseases, killing over a million people worldwide each year. The search for new dosage forms for the treatment of drug-resistant tuberculosis is an actual task. Biocompatible polymer nanoparticles, in particular bovine serum albumin (BSA), are promising drug carriers. Nanoparticle (NP) parameters such as diameter, polydispersity, bioactive substance loading, and NP yield are very important when it comes to drug transport through the bloodstream. The most well-known and widely used first-line anti-tuberculosis drug, isoniazid (INH), is being used as a drug. BSA-INH NPs were obtained by an ethanol desolvation of an aqueous protein solution in the drug presence. The peculiarity of the method is that natural components, namely urea and cysteine, are used for the stabilization of BSA-INH NPs after desolvation. The characteristics of the obtained BSA-INH NPs are significantly affected by the concentration of protein, isoniazid, urea, and cysteine in the solution. The aim of the present study is to investigate the concentration effect of the system reacting components on the parameters of the NPs that are obtained. We have chosen the concentrations of four reacting components, i.e., BSA, isoniazid, urea, and cysteine, as controlling factors and applied the Taguchi method to analyze which concentration of each component has an important effect on BSA-INH NPs characteristics.
This article considers someaspects of synthesis and characterizationof polylactide-co-glycolide nanoparticles immobilized withthe antituberculous drug isoniazid. The influence of some synthesis parameters of nanoparticles (the ratio of drug substance:polymer and surfactant concentration) onproperties of the obtained nanosomal drug form of isoniazid has been studied. Optimal conditions for obtainingthenanoparticles with the best physicochemical parameters such as: particle size, polydispersity, conversion, etc. have been found. These nanoparticlescan be used asdrug carriers.The results revealed thata polymer: drug ratio of 1:1 and the use of 3% Twin 80 are necessaryto obtain stable emulsions of nanoparticles of polylactide-co-glycolide with satisfactory characteristics. Average size of the obtained particles was 196.4 nm,and the polydispersity value was 0.323. The aggregation stability of nanoparticles during 4 hours at temperatures of 4ºC and 20ºC has been evaluated. The morphology of the obtained nanoparticles has been studied.Analysis of nanoparticles was characterized by various instrumental methods includinggas chromatography and thermogravimetrytechniques. The resulting nanoparticles of polylactide-co-glycolide immobilized with isoniazid are stable in time andcanprolong the action of the drug. In vitrorelease of isoniazid from polylactide-co-glycolide nanoparticles hasbeen studied.
The initial polypropylene glycol maleate has been obtained by the polycondensation reaction of maleic anhydride and propylene glycol. The molecular weight of the synthesized unsaturated polyester resin has been determined. The copolymers under study have been obtained by radical copolymerization of p-PGM. The possibility of synthesizing new polymers based on p-PGM with acrylic acid in the presence of a RAFT agent has been shown. The effect of RAFT agent concentration on the network density and product yield has been studied. It has been found that the yield of the cross-linked polymer decreases, its swelling capacity increases, and the yield of the linear polymer increases with an increase in the RAFT agent concentration. The effect of organic solvents, pH, and low-molecular-weight salts on the swelling degree of the synthesized copolymers has been investigated. The research results demonstrate that the susceptibility of polymer hydrogels to organic solvents and changes in pH, and low molecular weight salts, is affected by the amount of RAFT agent in the polymer. The synthesized objects have been characterized by infrared spectroscopy. Scanning electron microscopy has estimated the polymer surface morphology and pore sizes
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