Synthesis and characterization of isoniazid immobilized polylactide-co-glycolide nanoparticles

Abstract

This article considers someaspects of synthesis and characterizationof polylactide-co-glycolide nanoparticles immobilized withthe antituberculous drug isoniazid. The influence of some synthesis parameters of nanoparticles (the ratio of drug substance:polymer and surfactant concentration) onproperties of the obtained nanosomal drug form of isoniazid has been studied. Optimal conditions for obtainingthenanoparticles with the best physicochemical parameters such as: particle size, polydispersity, conversion, etc. have been found. These nanoparticlescan be used asdrug carriers.The results revealed thata polymer: drug ratio of 1:1 and the use of 3% Twin 80 are necessaryto obtain stable emulsions of nanoparticles of polylactide-co-glycolide with satisfactory characteristics. Average size of the obtained particles was 196.4 nm,and the polydispersity value was 0.323. The aggregation stability of nanoparticles during 4 hours at temperatures of 4ºC and 20ºC has been evaluated. The morphology of the obtained nanoparticles has been studied.Analysis of nanoparticles was characterized by various instrumental methods includinggas chromatography and thermogravimetrytechniques. The resulting nanoparticles of polylactide-co-glycolide immobilized with isoniazid are stable in time andcanprolong the action of the drug. In vitrorelease of isoniazid from polylactide-co-glycolide nanoparticles hasbeen studied.

Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method

“…The drug was released from BSA-INH-NPs within 24 h. The initial burst release after 2 h could be due to the desorption of the adsorbed INH from the surface of the particles, which is in agreement with the results observed in [29]. BSA-INH NPs have shown a sustained release for at least 72 h when compared to the free drug, which we studied earlier in [30], and the results here demonstrated almost 100% release within 5 h.…”

“…The drug was released from BSA-INH-NPs within 24 h. The initial burst release after 2 h could be due to the desorption of the adsorbed INH from the surface of the particles, which is in agreement with the results observed in [29]. BSA-INH NPs have shown a sustained release for at least 72 h when compared to the free drug, which we studied earlier in [30], and the results here demonstrated almost 100% release within 5 h. mg/mL. The drug was released from BSA-INH-NPs within 24 h. The initial burst release after 2 h could be due to the desorption of the adsorbed INH from the surface of the particles, which is in agreement with the results observed in [29].…”

Encapsulation of Isoniazid in Polylactide-Co-Glycolide Nanoparticles by Nanoprecipitation

“…The first phase is caused by the release of the drug substance, which is adsorbed on the surface of the NPs or dispersed near the surface. The second phase is due to the release of the drug substance residing in the core [28][29][30][31]. As shown in Figure 4, "burst release" of the drug is observed at pH 6.86 after 2 hours; at pH 7.4 after 5 hours; and at pH 1.2 was 27 % within an hour.…”

“…This may be because the solubility of isoniazid increases with pH decreasing. We have previously studied the release of a drug from a dialysis membrane in a conventional dosage form (the conventional isoniazid tablet), which provides a single and short-term release of the drug [29]. At pH = 1.2, the drug is released normally and the next doses of the drug are required to achieve a therapeutic effect [33,34].…”

Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles

“…Moreover, CCD was successfully used in our previous study to optimize and develop a method for the synthesis of HSA NPs loaded with anti-TB drugs, and the data produced by CCD showed good and reliable predictions [19]. The variables in Table 1 were selected based on our initial studies [11,20].…”