No abstract

No abstract

No abstract

No abstract

Cleavage of varicella-zoster virus DNA with the restriction endonucleases PstI, XbaI, and BglII resulted in 18, 22, and 20 fragments, respectively. Based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 106 could be calculated for the varicella-zoster virus genome. In both the XbaI and the BglII patterns, four 0.5 M fragments were identified. The arrangement of the fragments was determined by molecular hybridization techniques, and the terminal fragments were identified by A exonuclease digestion. The 0.5 M fragments, of which two were located at the same terminus of the genome, contained repeated sequences: one terminally and one inverted internally. These results were in agreement with the existence of two equimolar subpopulations of the varicella-zoster virus genome, differing in the relative orientation of a short region of unique sequences. This region was bounded by the repeated sequences. From the molecular weights of the submolar fragments, a maximal molecular weight of 5 x 106 for the repeated region and a minimal molecular weight of 3.5 x 106 for the short unique sequence could be calculated. MgCl2-50 mM NaCl-6 mM f8-mercaptoethanol; for XbaI, 6 mM Tris-hydrochloride (pH 7.9)-6 mM MgCl2-150 mM NaCl-6 mM ,B-mercaptoethanol; and for BglII, 10 mM Tris-hydrochloride (pH 7.4)-6 mM KCl-10 mM MgCl2-1 mM dithiothreitol. Each reaction mixture was incubated for 2 h at 37°C (XbaI and BglII) or 1 h at 30°C (PstI) with sufficient enzyme to 390