The results suggest that potato in vitro supports diverse bacterial endophytes. The community composition of the culturable component of the microflora was remarkably different from that revealed by culture-independent method. Introduction of Pseudomonas fluorescens IMBG163 into potato plant tissue resulted in essential rise of endophytic bacterial species number, however, in the further cloning their number was reduced. Endophytic isolates from potato varieties Zagadka and Nigru, induced by the rhizobacterium, exhibited features beneficial for plants.
A bacterial strain IMBG1S6 producing exopolysaccharide (EPS) was isolated from siliceous rock and identified as a Paenibacillus species by partial sequencing its 16S rDNA. Paenibacillus sp. IMBG156 was used in a novel technology for inoculant production based on co-cultivating this bacterium with any bacterium of choice. Paenibacillus sp. provides in situ the bacterial cells of the inoculant with EPS, a carrier, and most likely with a source of carbon and energy. The partner bacterium designates a type of inoculant (biopesticide or biofertiliser). The strain IMBG156 does not destroy the signaling system of Gram-negative partners, based on acylated homoserine lactones, stimulates plant growth, and is rather competitive in the plant rhizosphere and soil. A prototype of the inoculant based on dual-culturePaenibacillus sp. IMBG156 -Pseudomonas sp. IMBG163 exhibits a noticeably longer shelf life than monoculture of Pseudomonas sp. IMBGI63.
Создан эффективный и удобный при использовании биопрепарат для выращивания рас тений, безопасных для здоровья людей, на территориях, загрязненных тяжелыми металлами. Препа рат предотвращает поступление в растение токсичных элементов, защищает от болезней, стимулиру ет развитие растения в условиях дефицита питательных веществ. Препарат представляет собой кон сорциум бактерий с комплексом полезных для растений свойств, который обеспечивает его многоцелевое использование. Препарат был испытан в рамках комплексной программы его изучения в модельных и естественных агроценозах. Основные результаты испытания свидетельствуют о том, что бактерии препарата препятствуют накоплению тяжелых металлов в наземной части сои, тем са мым создавая условия для выращивания безопасного урожая и ремедиации почв. Ключевые слова: тяжелые металлы, техногенное загрязнение почвы, бактериальный препарат, био ремедиация.
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