A rationally assembled microbial community for growing Tagetes patula L. in a lunar greenhouse

Lytvynenko, Tetyana; Zaetz, I.; Voznyuk, T.; Kovalchuk, M. V.; Rogutskyy, I.S.; Mytrokhyn, O.; Lukashov, D. V.; Estrella-Liopis, Vitorio; Borodinova, Tetyana; Mashkovska, S.P.; Foing, B. H.; Kordyum, V. A.; Kozyrovska, Natalia

doi:10.1016/j.resmic.2005.07.009

Cited by 23 publications

(12 citation statements)

References 14 publications

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“…In other ground-based experiments, we were able to show that Paenibacillus sp. caused biocorrosion of anorthosite rock (Lytvynenko et al, 2006). In total, we can presume that a wide variety of different microorganisms, even from higher evolutionary advanced levels than those of archaea or bacteria, are able to resist and survive space and Mars-like conditions for a period of time (at least for 1.5 years).…”

Section: Results From Previous Spaceflight and Ground-based Experimentioning

confidence: 99%

Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS

et al. 2019

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BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the exposure facility EXPOSE-R2 outside the Zvezda module on the International Space Station (ISS). The design of the multiuser facility supports—among others—the BIOMEX investigations into the stability and level of degradation of space-exposed biosignatures such as pigments, secondary metabolites, and cell surfaces in contact with a terrestrial and Mars analog mineral environment. In parallel, analysis on the viability of the investigated organisms has provided relevant data for evaluation of the habitability of Mars, for the limits of life, and for the likelihood of an interplanetary transfer of life (theory of lithopanspermia). In this project, lichens, archaea, bacteria, cyanobacteria, snow/permafrost algae, meristematic black fungi, and bryophytes from alpine and polar habitats were embedded, grown, and cultured on a mixture of martian and lunar regolith analogs or other terrestrial minerals. The organisms and regolith analogs and terrestrial mineral mixtures were then exposed to space and to simulated Mars-like conditions by way of the EXPOSE-R2 facility. In this special issue, we present the first set of data obtained in reference to our investigation into the habitability of Mars and limits of life. This project was initiated and implemented by the BIOMEX group, an international and interdisciplinary consortium of 30 institutes in 12 countries on 3 continents. Preflight tests for sample selection, results from ground-based simulation experiments, and the space experiments themselves are presented and include a complete overview of the scientific processes required for this space experiment and postflight analysis. The presented BIOMEX concept could be scaled up to future exposure experiments on the Moon and will serve as a pretest in low Earth orbit.

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Section: Results From Previous Spaceflight and Ground-based Experimentioning

confidence: 99%

Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS

et al. 2019

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“…These organisms can be produced in large quantities, only requiring water, a bioreactor, and an easily transportable growth medium [5]. Microbes can be successfully used in many different techniques to produce [9], extract [10], and pattern [11] material in a manner that is similar to the equivalent mechanochemical processes. Microbial production processes do not necessarily require sophisticated factories, high energy investment, or toxic chemicals [4].…”

Section: Introductionmentioning

confidence: 99%

Iron can be microbially extracted from Lunar and Martian regolith simulants and 3D printed into tough structural materials

et al. 2021

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In-situ resource utilization (ISRU) is increasingly acknowledged as an essential requirement for the construction of sustainable extra-terrestrial colonies. Even with decreasing launch costs, the ultimate goal of establishing colonies must be the usage of resources found at the destination of interest. Typical approaches towards ISRU are often constrained by the mass and energy requirements of transporting processing machineries, such as rovers and massive reactors, and the vast amount of consumables needed. Application of self-reproducing bacteria for the extraction of resources is a promising approach to reduce these pitfalls. In this work, the bacterium Shewanella oneidensis was used to reduce three different types of Lunar and Martian regolith simulants, allowing for the magnetic extraction of iron-rich materials. The combination of bacterial treatment and magnetic extraction resulted in a 5.8-times higher quantity of iron and 43.6% higher iron concentration compared to solely magnetic extraction. The materials were 3D printed into cylinders and the mechanical properties were tested, resulting in a 400% improvement in compressive strength in the bacterially treated samples. This work demonstrates a proof of concept for the on-demand production of construction and replacement parts in space exploration.

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“…These organisms can be produced in large quantities, only requiring water and an easily transportable growth medium [5]. Microbes can be successfully used in many different techniques to produce [9], extract [10], and pattern [11] material in a manner that is similar to the equivalent mechanochemical processes. Microbial production processes do not require sophisticated factories, high energy investment, or toxic chemicals [4].…”

Section: Introductionmentioning

confidence: 99%

Iron can be microbially extracted from Lunar and Martian regolith simulants and 3D printed into tough structural materials

Castelein

Aarts

Schleppi

et al. 2020

Preprint

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In-situ resource utilization (ISRU) is increasingly acknowledged as an essential requirement for the construction of sustainable extra-terrestrial colonies. Even with decreasing launch costs, the ultimate goal of establishing colonies must be the usage of resources found at the destination of interest. Typical approaches towards ISRU are often constrained by the mass and energy requirements of transporting processing machineries, such as rovers and massive reactors, and the vast amount of consumables needed. Application of self-reproducing bacteria for the extraction of resources is a promising approach to avoid these pitfalls. In this work, the bacterium Shewanella oneidensis was used to reduce three different types of Lunar and Martian regolith simulants, allowing for the magnetic extraction of iron-rich materials. The quantity of bacterially extracted material was up to 5.8 times higher and the total iron concentration was up to 43.6% higher in comparison to untreated material. The materials were 3D printed into cylinders and the mechanical properties were tested, resulting in a 396 ± 115% improvement in compressive strength in the bacterially treated samples. This work demonstrates a proof of concept for the on-demand production of construction and replacement parts in space exploration.

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A rationally assembled microbial community for growing Tagetes patula L. in a lunar greenhouse

Cited by 23 publications

References 14 publications

Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS

Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS

Iron can be microbially extracted from Lunar and Martian regolith simulants and 3D printed into tough structural materials

Iron can be microbially extracted from Lunar and Martian regolith simulants and 3D printed into tough structural materials

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