Paenibacillus sp., as a promising candidate for development of a novel technology of inoculant production

Kozyrovska, Natalia; Negrutska, V. V.; Kovalchuk, M. V.; Voznyuk, T.

doi:10.7124/bc.0006f7

Cited by 9 publications

(4 citation statements)

References 21 publications

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“…‘helper’ strain was co‐cultivated with Gram‐negative biocontrol and biofertilizer strains ( K. oxytoca, Pseudomonas sp ., P. fluorescens, P. aureofaciens, P. agglomerans, Agrobacterium sp.) (Kozyrovska et al ., ). These combinations proved to be compatible for co‐cultivation, i.e.…”

Section: Desiccation Damage and Protective Strategiesmentioning

confidence: 97%

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Berninger

López

Bejarano

et al. 2017

Microbial Biotechnology

153

106

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SummaryThe application of beneficial, plant‐associated microorganisms is a sustainable approach to improving crop performance in agriculture. However, microbial inoculants are often susceptible to prolonged periods of storage and deleterious environmental factors, which negatively impact their viability and ultimately limit efficacy in the field. This particularly concerns non‐sporulating bacteria. To overcome this challenge, the availability of protective formulations is crucial. Numerous parameters influence the viability of microbial cells, with drying procedures generally being among the most critical ones. Thus, technological advances to attenuate the desiccation stress imposed on living cells are key to successful formulation development. In this review, we discuss the core aspects important to consider when aiming at high cell viability of non‐sporulating bacteria to be applied as microbial inoculants in agriculture. We elaborate the suitability of commonly applied drying methods (freeze‐drying, vacuum‐drying, spray‐drying, fluidized bed‐drying, air‐drying) and potential measures to prevent cell damage from desiccation (externally applied protectants, stress pre‐conditioning, triggering of exopolysaccharide secretion, ‘helper’ strains). Furthermore, we point out methods for assessing bacterial viability, such as colony counting, spectrophotometry, microcalorimetry, flow cytometry and viability qPCR. Choosing appropriate technologies for maintenance of cell viability and evaluation thereof will render formulation development more efficient. This in turn will aid in utilizing the vast potential of promising, plant beneficial bacteria as sustainable alternatives to standard agrochemicals.

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Section: Desiccation Damage and Protective Strategiesmentioning

confidence: 97%

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Berninger

López

Bejarano

et al. 2017

Microbial Biotechnology

153

106

View full text Add to dashboard Cite

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“…Relating to the current research, the co-culture of exopolysaccharide-producing Paenibacillus sp. with a Pseudomonas species has been reported to extend the shelf-life of the Pseudomonas for potential biopesticide or biofertilizer use (Kozyrovska, Negrutska, Kovalchuk, and Voznyuk 2005). Thus, in addition to enhancing its biocontrol capacity, one of the benefits of our co-culture is that it includes an EPS-productive partner in P. fluorescens strain S11:P:12 that may protect bacteria against desiccation stress.…”

Section: Discussionmentioning

confidence: 98%

Multi-strain co-cultures surpass blends for broad spectrum biological control of maladies of potatoes in storage

Slininger

Schisler

Shea-Andersh

et al. 2010

Biocontrol Science and Technology

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Pseudomonas fluorescens strains S11:P:12, P22:Y:05, and S22:T:04 and Enterobacter cloacae strain S11:T:07 have been documented to suppress four important storage potato maladies Á dry rot, late blight, pink rot, and sprouting. This research investigates the efficacy and consistency of strain mixtures produced by co-culturing strains together in one vessel or by blending them together after separate cultivations in pure cultures. Pure and co-cultures were produced in flask or fermentor cultures, viable cell concentrations were assessed using a nutrientbased selective plating method to identify and enumerate strains, and the efficacy of treatments was assessed with respect to dry rot, pink rot, late blight or sprout suppression. Experiments were designed to analyze dry rot suppression versus all strain combinations and the combination method (co-culture or blend). Results of a two-way analysis of variance of disease with strain composition and combination method showed that significantly better dry rot suppression was obtained by co-cultures (30.392.4% relative disease) than by similar strain blends of pure cultures (41.392.4%) (P B0.001). During a 3-year study, both biocontrol efficacy and consistency were assessed in 16 laboratory and small pilot trials simulating commercial storages. Three-strain co-culture had a lower mean disease rating than the blend in 9 of 16 experiments examining control of the three diseases and sprouting. The co-culture led other treatments in incidences of significant malady reduction relative to the control: 14 of 16 attempts for coculture, 11 of 16 attempts for blend, 10 of 13 attempts for pure S11:P:12, 8 of 13 attempts for S22:T:04, and 9 of 13 attempts for P22:Y:05. Using relative performance indices to rank treatment performance across all experiments, the co-culture treatment ranked significantly higher than the blend. A synergy analysis suggested that co-culturing strains stimulated inter-strain activities to boost biocontrol efficacy and consistency, a feature not developed in strains grown separately and mixed just prior to addition to potatoes.

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“…The co-culture of exopolysaccharideproducing Paenibacillus sp. with a Pseudomonas species has been reported to extend the shelf-life of the Pseudomonas for potential biopesticide or biofertilizer use (Kozyrovska, Negrutska, Kovalchuk, and Voznyuk 2005). Similarly, the three biocontrol strains of P. fluorescens studied here are also known to function most consistently to reduce biocontrol when applied as mixtures (Schisler et al 1997;Slininger et al 2001Slininger et al , 2007.…”

Section: The Role Of Marginalanmentioning

confidence: 90%

Polysaccharide production benefits dry storage survival of the biocontrol agentPseudomonas fluorescensS11:P:12 effective against several maladies of stored potatoes

Slininger

Dunlap

Schisler

2010

Biocontrol Science and Technology

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Pseudomonas fluorescens S11:P:12 (NRRL B-21133) is a biological control agent able to suppress several potato diseases and sprouting. Notably, it produces a polysaccharide during liquid cultivation, and the objective of this work was to determine the role of this material in the bio-control process. First, the polysaccharide was isolated, purified and identified as marginalan, which accumulated to Â3.3 g/L in cultures. The bioactivity of isolated marginalan applied alone or in combination with washed cells of strain S11:P:12 was tested in potato bioassays of dry rot and pink rot suppressiveness and sprout inhibition. Since the formulation and storage of a dried biocontrol product is preferred for commercial use, the impact of marginalan on cell survival during drying and storage was also studied. Washed bacteria formulated with 0Á6.6 g/L polysaccharide were either applied to Hyflo granules, then slowly dried for 24 h with airflow at 50Á60% relative humidity, or in 1-mL droplets placed in replicate wells of a micro-plate, then quickly dried for 1 h in a biohazard hood. Both Hyflo and micro-plate dry storage results indicated that marginalan significantly reduced cell death after drying, such that the final stable viable cell density was 2.5Á5 orders of magnitude greater, respectively, than if no marginalan were included with cells. Marginalan had no significant impact on disease or sprout suppression by strain S11:P:12, and its main benefit to biocontrol was viable cell preservation during drying and storage. When marginalan was formulated with other selected P. fluorescens strains, its benefits to drying and storage survival were again evident (especially after 48C instead of 258C storage), but its effects were more subtle than for strain S11:P:12, and dry rot suppression was not impacted.

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Paenibacillus sp., as a promising candidate for development of a novel technology of inoculant production

Cited by 9 publications

References 21 publications

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Maintenance and assessment of cell viability in formulation of non‐sporulating bacterial inoculants

Multi-strain co-cultures surpass blends for broad spectrum biological control of maladies of potatoes in storage

Polysaccharide production benefits dry storage survival of the biocontrol agentPseudomonas fluorescensS11:P:12 effective against several maladies of stored potatoes

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