Heavy metal pollution in water is a serious environmental concern. Lead is one of the highly toxic heavy metals, and its toxicity is due to its non-degradation characteristic in living organisms. The monitoring of Pb(II) ions concentrations in drinking water is essential for human health and a safe environment. In this work, bio-modified carbon paste electrodes were constructed with live cells and thermally dried biomass (extracted protein) from Pseudomonas aeruginosa cells and employed for the detection of Pb(II) ions from wastewater. The biomass and biosensor preparation and optimization, and the performance of modified biosensors in the detection of Pb(II) ions are discussed here. The effect of various parameters, like pH, biomass composition, scan rate, and metal ion concentration, were studied to obtain the best electrochemical response. Further, the active surface of the electrode and solution characteristics were studies using chronocoulometry and electrochemical impedance spectroscopy. Fourier transform infrared spectroscopy (FTIR) analysis was done to find the functional groups present in the thermally dried biomass. From the present study, it is clear that the thermally dried protein biomass electrode has more sensitivity than the bare carbon paste electrode.
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The technique of neutral zone is useful. It is the convergence of many concepts and ideas into a feasible and functional method. The technique aims to build a denture in accordance with the underlying oral structures that are formed by muscle function. It acts as an alternative technique in case of highly atrophic ridges. With a history of denture instability, it is most successful. This paper seeks to provide information with an enclosure of a patient input study to use the technique.
Cleft lip and palate is one of the most common birth defect. Rehabilitation of such defects requires a multidisciplinary approach. Treatment can range from simple removable appliances to fixed prosthesis using implants. The size of the defect, age and socioeconomic status influences the treatment planning. This case report describes a conservative approach to rehabilitate a maxillary defect with missing teeth using a heat polymerized acrylic resin improving the function, esthetics and speech.
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