A RPLC method with UV detection (225 nm) is developed for the separation of five SSRIs (fluvoxamine, fluoxetine, sertraline, paroxetine, and citalopram), two SNaRIS (venlafaxine and milnacipran), one NaSSA (mirtazapine), and four active metabolites (norfluoxetine, desmethylcitalopram, desmethylvenlafaxine, and desmethylmirtazapine). A standard solution (20 mg=mL) of the twelve compounds is analysed under isocratic conditions on two new-generation RP columns (Satisfaction 1 RP 18 AB and Satisfaction 1 C8þ , 250 mm64.6 mm, 5 mm). Mobile phase composition (acetonitrile content, pH of the aqueous buffer) and temperature are varied and the effect of these parameters on the retention factors of the antidepressants is examined. Similar ORDER REPRINTS elution profiles are observed with the two stationary phases, but the separation of all the solutes is only possible on the RP 18 AB column. It can be achieved at 45 C (or 50 C) with a mobile phase consisting of a mixture of potassium dihydrogen phosphate (pH 4.8, 25 mM)-acetonitrile (65:35, v=v) (flow rate: 1 mL=min). The run time is 20 min and a baseline resolution is obtained for all the analytes allowing this procedure to be well suited for a rapid toxicological screening.
In recent years there has been a renaissance in thin-layer chromatography (TLC) with the introduction of high-performance plates, sophisticated spectrophotometric TLC scanners, and novel spotting techniques. An important problem that remains to be solved both in high-performance liquid chromatography and in TLC is the development of a logical method for optimizing the solvent composition of the
AU,, the separation between a pair of compounds on a thin layer chromatographic plate, can be predicted as a function of solvent composition for certain binary systems. This allows the prediction of optimum solvent composition for separating a mixture of compounds by thin layer chromatography. A new solvent polarity ranking, based on calculation of AR, is described.
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