A Reversed-Phase HPLC Method Development for the Separation of New Antidepressants

Dallet, P.; Labat, Laurence; Richard, M.; Langlois, Michel; Dubost, Jean‐Pierre

doi:10.1081/jlc-100108542

Cited by 23 publications

(10 citation statements)

References 18 publications

(5 reference statements)

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“…The determination of STR in serum and plasma is generally performed by high performance liquid chromatography (HPLC) coupled with spectrophotometric detectors, ultraviolet/diode array [1][2][3][4][5][6] and fluorimetric [7,8]. The wide spread usage of HPLC methods is justified by the high sensitivity and low limit of detection usually obtained and the possibility of the simultaneous analysis of the drug and its metabolites, which increases its usage even further.…”

Section: Introductionmentioning

confidence: 99%

Electroanalytical study of the antidepressant sertraline

Nouws

Delerue–Matos

Barros

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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A flow injection square wave cathodic stripping voltammetric method has been developed for the determination of sertraline in a pharmaceutical preparation. The method shows linearity between peak current intensity and sertraline concentration for the interval between 0.20 × 10 −6 and 1.20 × 10 −6 mol L −1 . Limits of detection and quantification were found to be 1.5 × 10 −7 and 5.0 × 10 −7 mol L −1 , respectively. Up to 70 samples per hour can be analysed with a good precision (R.S.D. = 2.5%). The proposed method was successfully applied to the determination of sertraline in a commercial product. In the voltammetric determination of sertraline in flow, a high sample rate is obtained at reduced costs, opening the possibility to compete with the chromatographic methods generally used for this analysis.

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Section: Introductionmentioning

confidence: 99%

Electroanalytical study of the antidepressant sertraline

Nouws

Delerue–Matos

Barros

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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“…Most FVX analysis is performed by high-perfor-mance liquid chromatography (HPLC) coupled with spectrometric detectors, ultraviolet/diode array [4][5][6][7][8][9][10][11][12][13][14][15][16][17], mass spectrometric [18,19] and fluorimetric [20][21][22], for analysis of pharmaceuticals [8] and human serum or plasma [4][5][6][7][10][11][12][13][14][15][16][17][18][19][20][21][22]. This widespread usage of HPLC methods is justified by the high sensitivity and the low limit of detection usually obtained and the possibility of simultaneous analysis of the drug and its metabolites, which increases its use even further.…”

Section: Introductionmentioning

confidence: 99%

Electroanalytical study of fluvoxamine

et al. 2005

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Fluvoxamine (FVX) can be reduced at a mercury-drop electrode, with a maximum peak current intensity being obtained at a potential of -0.7 V vs. Ag/ AgCl, in an aqueous electrolyte solution of pH 2. The compound was determined in a pharmaceutical product and in spiked human serum by square-wave adsorptivestripping voltammetry (SWAdSV) after accumulation at the electrode surface, under batch conditions. Because the presence of dissolved oxygen did not interfere significantly with the analysis, it was also possible to determine FVX in the pharmaceutical product by use of a flow-injection analysis (FIA) system with SWAdSV detection. The methods developed were validated and successfully applied to the quantification of FVX in a pharmaceutical product. Recoveries between 76 and 89% were obtained in serum analysis. The FIASWAdSV method enabled analysis of up to 120 samples per hour at reduced cost, implying the possibility of competing with the chromatographic methods usually used for this analysis.

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“…Only some nonchiral methods for the analysis of CIT and other SSRI drugs by HPLC-UV [35,36] CZE [37,38], and isotachophoresis [39] are available. We have implemented with this study a fast CZE stereoselective separation of CIT which could be applied to the determination of the enantiomeric purity of preparations containing either racemic or chirally resolved CIT.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric separation of citalopram and its metabolites by capillary electrophoresis

et al. 2003

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A simple and fast capillary electrophoretic method has been developed for the enantioselective separation of citalopram and its main metabolites, namely N-desmethylcitalopram and N,N-didesmethylcitalopram, using beta-cyclodextrin (beta-CD) sulfate as the chiral selector. For method optimisation several parameters were investigated, such as CD and buffer concentration, buffer pH, and capillary temperature. Baseline enantioseparation of the racemic compounds was achieved in less than 6 min using a fused-silica capillary, filled with a background electrolyte consisting of a 35 mM phosphate buffer at pH 2.5 supplemented with 1% w/v beta-CD sulfate and 0.05% w/v beta-CD at 25 degrees C and applying a voltage of -20 kV. A fast separation method for citalopram was also optimized and applied to the analysis of pharmaceutical formulations. Racemic citalopram was resolved in its enantiomers in less than 1.5 min using short-end injection (8.5 cm, effective length) running the experiments in a background electrolyte composed of a 25 mM citrate buffer at pH 5.5 and 0.04% w/v beta-CD sulfate at a temperature of 10 degrees C.

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A Reversed-Phase HPLC Method Development for the Separation of New Antidepressants

Cited by 23 publications

References 18 publications

Electroanalytical study of the antidepressant sertraline

Electroanalytical study of the antidepressant sertraline

Electroanalytical study of fluvoxamine

Enantiomeric separation of citalopram and its metabolites by capillary electrophoresis

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