Determination of fumagillin in muscle tissue of rainbow trout using automated ion-pairing liquid chromatography

Guyonnet, J.; Richard, M.; Hellings, Ph.

doi:10.1016/0378-4347(94)00577-r

Cited by 10 publications

(7 citation statements)

References 9 publications

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“…SPE based sample treatments have also been applied to the analysis of fumagillin in fish tissue, but due to the nature of this matrix, an additional sample treatment step is necessary. Hence, Guyonnet et al [59] proposed a method which includes a sample extraction with water and acetonitrile prior to SPE. Fekete et al [60] developed two sample treatments that could be used, depending on the sensitivity requirements.…”

Section: Analytical Methods For the Determination Of Fumagillinmentioning

confidence: 99%

“…The latter was used by Nozal et al [53] for the determination of fumagillin and is especially interesting because at this pH value (6.5) the carboxylic group is deprotonated. For this reason, the acidic nature of fumagillin was employed by Guyonnet et al [59] to develop a method based on ion-pairing. A mobile phase at pH 7.8 was used, where tetrabutylammonium acts as a cation to retain fumagillin in a C8 column.…”

Section: Analytical Methods For the Determination Of Fumagillinmentioning

confidence: 99%

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Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Guruceaga

Perez-Cuesta

Abad‐Diaz‐de‐Cerio

et al. 2019

Toxins

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Fumagillin is a mycotoxin produced, above all, by the saprophytic filamentous fungus Aspergillus fumigatus. This mold is an opportunistic pathogen that can cause invasive aspergillosis, a disease that has high mortality rates linked to it. Its ability to adapt to environmental stresses through the production of secondary metabolites, including several mycotoxins (gliotoxin, fumagillin, pseurotin A, etc.) also seem to play an important role in causing these infections. Since the discovery of the A. fumigatus fumagillin in 1949, many studies have focused on this toxin and in this review we gather all the information currently available. First of all, the structural characteristics of this mycotoxin and the different methods developed for its determination are given in detail. Then, the biosynthetic gene cluster and the metabolic pathway involved in its production and regulation are explained. The activity of fumagillin on its target, the methionine aminopeptidase type 2 (MetAP2) enzyme, and the effects of blocking this enzyme in the host are also described. Finally, the applications that this toxin and its derivatives have in different fields, such as the treatment of cancer and its microsporicidal activity in the treatment of honeybee hive infections with Nosema spp., are reviewed. Therefore, this work offers a complete review of all the information currently related to the fumagillin mycotoxin secreted by A. fumigatus, important because of its role in the fungal infection process but also because it has many other applications, notably in beekeeping, the treatment of infectious diseases, and in oncology. Key Contribution:Fumagillin is a mycotoxin produced during infections caused by A. fumigatus and in the adaptation processes of this species to multiple environmental stresses. In this review; we include detailed information on the fungal mechanisms employed in the production of this mycotoxin and those present in its regulation, how it acts on its target, and how it can be detected, all of which may be useful for diagnosis or the development of new treatments. Its usefulness in different fields is also described; because of its anti-angiogenic activity it is used to attack different types of tumors; at the same time its antibiotic activity is useful against several parasites.

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Section: Analytical Methods For the Determination Of Fumagillinmentioning

confidence: 99%

Section: Analytical Methods For the Determination Of Fumagillinmentioning

confidence: 99%

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Guruceaga

Perez-Cuesta

Abad‐Diaz‐de‐Cerio

et al. 2019

Toxins

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“…Current analytical methods to detect fumagillin in honey only recognize fumagillin and its UV, thermal and hydrolysis degradation products (Figure 1), without recognizing the importance of DCH (Gochnauer and Furgala 1962;Brackett et al 1988;Assil and Sporns 1991;Guyonnet et al 1995;Kochansky and Nasr 2004;Lopez et al 2008;Higes et al 2011;Kanda et al 2011;Nozal et al 2008;Dmitrovic and Durden 2013). DCH is, however, briefly mentioned as a component of the commercial formulation Fumgilin-B, that is detectable by LC-MS/MS methodology, but without recognizing its importance as a potential chemical contaminant in honey (Dmitrovic and Durden 2013).…”

Section: Introductionmentioning

confidence: 99%

Stability of dicyclohexylamine and fumagillin in honey

et al. 2015

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“…Fumagillin (Fig. 1) is an antibiotic effective against sporidiae, which was used in adult honeybees and in fish (Guyonnet et al. , 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Fumagillin (Fig. 1) is an antibiotic effective against sporidiae, which was used in adult honeybees and in fish (Guyonnet et al, 1995). Fumagillin is no longer available on the EU market since January 2000 but is still available in the USA to treat nosema disease in bees.…”

Section: Introductionmentioning

confidence: 99%

Fumagillin, a new P‐glycoprotein‐interfering agent able to modulate moxidectin efflux in rat hepatocytes¹

Dupuy

Lespine

Sutra

et al. 2006

Vet Pharm & Therapeutics

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We have tested the ability of two compounds licensed in veterinary medicine: fumagillin and diminazene diaceturate to increase intracellular moxidectin quantity in rat hepatocytes. These compounds significantly increased the quantity of 14C-moxidectin (expressed as area under the time curve concentrations) in cultured rat hepatocytes by 44% and 65% for diminazene and fumagillin treatments respectively. In addition, we have tested these drugs for their interference with P-glycoprotein (P-gp) function in porcine kidney epithelial cells transfected with murine mdr1a (Mdr1a-LLCPK1). We examined the intracellular accumulation of rhodamine 123 (Rho 123) as a functional test to evaluate the effects of these two drugs on P-gp activity. In this model, only fumagillin led to a marked intracellular accumulation of Rho 123. After transforming the data to express the results as a percentage of the accumulation in the presence of the P-gp inhibitor valspodar (VSP), the maximal Rho 123 accumulation was 47% of that with VSP for 100 microm fumagillin. The EC50, the concentration needed to determine 50% of the maximal effect was 34 microm. Fumagillin interacts with P-gp function and appears as a promising compound among registered drugs available, which may optimize the therapeutic use of macrocyclic lactones (MLs).

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Determination of fumagillin in muscle tissue of rainbow trout using automated ion-pairing liquid chromatography

Cited by 10 publications

References 9 publications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Stability of dicyclohexylamine and fumagillin in honey

Fumagillin, a new P‐glycoprotein‐interfering agent able to modulate moxidectin efflux in rat hepatocytes¹

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Determination of fumagillin in muscle tissue of rainbow trout using automated ion-pairing liquid chromatography

Cited by 10 publications

References 9 publications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Stability of dicyclohexylamine and fumagillin in honey

Fumagillin, a new P‐glycoprotein‐interfering agent able to modulate moxidectin efflux in rat hepatocytes1

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Fumagillin, a new P‐glycoprotein‐interfering agent able to modulate moxidectin efflux in rat hepatocytes¹