The legend for figure 10 on page 373 does not agree with the orientation of the photograph. To read correctly, the photo should be turned 90 degrees clockwise with the rule at the bottom.Hybrids of 'Eureka' and 'Lisbon' lemons were studied along with their parents and possible ancestral types to investigate the phylogeny of Citrus Zintort (L.) Burm. f. Data on leaf, flower, and fruit characters were collected and analyzed for inheritance of specific characters and for the amount of variability. Leaf and rind-oil components were analyzed by gas chromatography. Those hybrids producing suscient microspores were studied for meiotic abnormalities. Segregation of all characters studied demonstrates the heterozygosity of C. limo*. Color (anthocyanin) development in flower buds and young leaves is most likely controlled by a single dominant gene. Color on the young stems may be controlled by two dominant genes, one of which also controls color in flower buds and young leaves. Preformed root initials are most likely controlled by one, and leaf articulation by at least two genes.Characters specific to C. medica were present in the lemon progeny, indicating definite involvement of the citron in the lemon ancestry. Some characters indicate a possible common gene source with C. amadfolk and C. jambhiri. The deviation of some hybrids from their parents or proposed ancestors indicates a contributing gene source that has not yet been identified.The presence of much sterility and wide segregation of characters argues against the specific standing of C. & m m . It has a common gene source with the citron-lime group, and it may be a derived hybrid involving some unidentified taxa. The overlapping geographical distribution of various Citrus taxa at the assumed place of origin of C. 1imo.n further suggests the possibility of intercrowing or common origin of various Citrus species.
Alternative procedures for the production of Narcissus L. somatic embryos were investigated. Somatic embryogenesis was initiated on ovary explants isolated from cv. Carlton bulbs, chilled for 12 weeks at 5°C. The explants were cultured on MS media with 3% sucrose and growth regulators: Picloram or 2,4-D (10 or 25 lM) and BA (1 or 5 lM) for 12 weeks in the culture systems: continuous cultivation on solid media, continuous cultivation in liquid media and sequential cultivation using cycles in liquid and solid media. Two types of somatic embryogenesis, indirect and direct, were observed. The developmental pathway depended on the period of exposure to liquid media. Somatic embryos were formed via embryogenic nodular callus on solid media. 2,4-D and BA stimulated the process. The 4-week and 8-week liquid medium treatments resulted in the development of somatic embryos directly from the ovary explant tissue. The highest number of somatic embryos was noted under the influence of 25 lM 2,4-D and 5 lM BA in explants cultivated for 8 weeks in liquid medium and then, for 4 weeks, on solid medium. The effects of inoculum density on biomass increase and the formation of somatic embryos in cultures obtained on a medium with 25 lM 2,4-D and 5 lM BA were also checked. The highest biomass increase was observed after subculturing in liquid medium containing 0.5 lM NAA and 5 lM BA when the density of inoculum was 0.5 g/25 ml of the medium. The highest number of somatic embryos was noted when the density of inoculum was 1.5 g/25 ml.
Wild roses are an important group of plants used as decorative shrubs and cut flowers. They are also a row material for pharmaceutical, cosmetic and food industries. For rose in vitro propagation, solid media are commonly used. Up till now there is a few reports confirming the beneficial effect of liquid media on Rosa shoot growth and multiplication. The aim of the study was to investigate different culture systems, temporary immersion system (TIS) (immersion frequencies of 15 min every 6, 8 and 12 h), rotary shaker (RS) and stationary liquid (SL) for propagation of R. canina and compare with solid medium culture. Shoot tips and stem explants were grown on basic Murashige and Skoog medium with 20 mg dm-3 Fe EDDHA, 1 µM BA, 1.5 µM GA3 and 3% sucrose for six weeks. Liquid cultures stimulated biomass growth. The highest biomass growth in RS cultures were observed however, RS reduced the shoot dry mass content. TIS cultures immersed every 6 and 8 h increased dry mass content. In TIS and on solid medium shoot multiplication was 1.5-2 times better than in other systems and stem explants were more efficient. Solid medium improved the content of chlorophyll a, b, a+b and carotenoids. Higher contents of photosynthetic pigments were determined in shoot tips than stem explants. TIS-derived plantlets accumulated the largest amount of phenolic compounds. As the frequency of immersion increases the concentration of these compounds were reduced. In turn, SL cultures favored the accumulation of soluble sugars.
JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. International Association for Plant Taxonomy (IAPT)is collaborating with JSTOR to digitize, preserve and extend access to Taxon. SummaryThe rough lemon group was investigated as to its coherence and affinity to the true lemon group. The probable affiliation of the Moroccan rough lemon was scrutinized. The possible origin of the true lemons were studied with the help of genetic and essential oil analysis. A divergence diagram of these groups and of a few old-line species is presented based upon an essential oil discriminant computer analysis.
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