Wharton’s Jelly is one of the best sources for mesenchymal stem cells. Human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSCs) have high proliferation, multi-lineage differentiation potential, and do not produce any teratogen or carcinogen. These characteristics make hWJ-MSCs become suitable for regenerative medicine. Some methods were developed to isolate hWJ-MSCs from umbilical cord, such as explant method and enzymatic method. This study aims to characterize hWJ-MSCs which are isolated by two different methods, explant attachment method and enzymatic method. hWJ-MSCs isolation was performed through explant method and enzymatic method using trypsin, hyaluronidase and collagenase type 1 with certain ratio of concentration. Isolated hWJ-MSCs was characterized using flow cytometer to detect the expression of CD44, CD90, CD105, CD73 and negative lineage. MSCs differentiation assay was performed to analyze adipogenic, chondrogenic and osteogenic cells lineage. We successfully isolated hWJ-MSCs from umbilical cord through enzymatic and explant methods. Immunophenotyping assay through flow cytometry analysis showed high purity of WJ-MSCs. The isolated hWJ-MSCs from both methods showed positive expression of CD44, CD90, CD105, and CD73. The isolated hWJ-MSCs exhibited capacity to differentiate into adipocyte, chondrocyte, and osteocyte cells. hWJ-MSCs isolated through explant and enzymatic method have high proliferation capacity and be able to differentiate into three different lineage cells. Both methods explant attachment and enzymatic methods are efficiently produced hWJ-MSCs.
Latar belakang: Usaha Gorengan Bunda merupakan home industry yang bekerja dengan cara Manual Material Handling (MMH) selama proses produksinya. Mengingat aktivitas MMH mempunyai peranan vital, maka perlu dilakukan penelitian untuk mengidentifikasi dan menganalisis postur pekerja. Penelitian ini bertujuan untuk menganalisis postur kerja menggunakan metode Rapid Upper Limb Assesment (RULA) pada pekerja Usaha Gorengan Bunda yang berlokasi di Siteba.Metode: Metode RULA merupakan metode observasi untuk menganalisis postur kerja tubuh bagian atas. Pengambilan data diperoleh melalui observasi lapangan untuk memperoleh skor RULA, pengisian kuesioner Nordic Body Map (NBM) untuk memperoleh data keluhan pekerja, dan data korelasi variabel usia dan masa kerja terhadap postur kerja yang digunakan.Hasil: Hasil penelitian menunjukkan bahwa pekerja pada proses membuat kulit gorengan dan proses membuat isi gorengan berada pada level action 4 yang berarti perlu dilakukan perubahan. Pada pekerja proses mengisi gorengan dan mengemas cabe berada pada level action 3 yang berarti diperlukan investigasi, perubahan diperlukan segera, sedangkan, pada proses mempersiapkan bahan berada pada level action 2 yang berarti investigasi diperlukan, perubahan mungkin diperlukan. Pada penelitian ini diperoleh korelasi antara usia dan masa kerja terhadap postur kerja pekerja dengan nilai signifikansi (p) sebesar 0,06 dan 0,039.Simpulan: Penelitian ini menunjukkan masa kerja mempunyai hubungan terhadap risiko postur kerja (p < 0,05). Tindakan perbaikan yang dapat dilakukan yaitu dengan menambah fasilitas kerja seperti menyediakan kursi yang memiliki penyangga punggung, memperbaiki fasilitas kerja sesuai dengan antropometri tubuh pekerja, melakukan kebiasaan berolahraga untuk menjaga kebugaran tubuh.Kata kunci: Industri; Postur Kerja; Ergonomi; Rapid Upper Limb Assesment (RULA); Nordic Body Map (NBM) ABSTRACT Title: Work Posture Analysis in Gorengan Home Industry using Rapid Upper Limb Assessment (RULA) MethodBackground: Gorengan Bunda Business is a home industry that works by means of Manual Material Handling (MMH) during the production process. Given that MMH activities have a vital role, it is necessary to conduct research to identify and analyze worker postures. This study aims to analyze work posture using the Rapid Upper Limb Assessment (RULA) method on the workers of the Gorengan Bunda Business located in Siteba. Method: The RULA method is an observational method for analyzing the work posture of the upper body. Data collection was obtained through field observations to obtain RULA scores, filling in the Nordic Body Map (NBM) questionnaire to obtain worker complaint data, and data on the correlation of age and working period variables to the work posture used. Result: The results showed that workers in the process of making fried skin and in the process of making fried fillings were at action level 4, which means that changes need to be made. In the process of filling the fry and packing chilies it is at action level 3 which means investigation is needed, changes are needed immediately, meanwhile, in the process of preparing ingredients it is at action level 2 which means investigation is needed, changes may be needed. In this study, it was found that the correlation between age and years of service on the work posture of workers with a significance value (p) of 0.06 and 0.039. Conclusion: This study shows that tenure has a relationship with the risk of work posture (p <0.05). Corrective actions that can be taken are adding work facilities such as providing chairs that have back support, improving work facilities according to the worker's body anthropometry, doing exercise habits to maintain body fitness.Keywords: Industry; Work Posture; Ergonomy; Rapid Upper Limb Assesment (RULA); Nordic Body Map (NBM)
In the process of in vitro culture, mesenchymal stem cells [MSC] release some secretions, resulting in a variety of growth factors and cytokines in the growth medium of MSCs. These media are called conditioned media [CM] and are currently considered to be a better alternative to stem cell therapy. To increase the potential of CM-MSCs in regenerative therapy, in this study we induced the SM-MSCs with IGF-1 to promotes growth factors and cytokine release in CM-SMMSCs. After SM-MSCs were treated with IGF-1, the CM was collected and BMP-1, FGF-18, and TGF-β1 were analyzed using ELISA method. Based on the results, the most significant increases in BMP-2, FGF18, and TGF-β1 protein concentrations were found in SM-MSCs-CM cultured with 150 ug / mL of IGF-1. Therefore, 150 μg / ml of IGF-1 is a potential concentration used for induced growth factors released in CM-SMMSCs.
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