This study was carried out in the tissue culture laboratory, Faculty of Agriculture, Saba basha, Alexandria University, Egypt during the period from 2013 to 2015. An efficient and reliable protocol for in vitro propagation of Magnolia grandiflora, Linn was optimized. However, nodal explants from field grown of magnolia were used during in vitro culture study for induction of multiple shoots. Nodal explants were effectively surface sterilized with 30% Clorox (sodium hypochlorite) as commercial bleaches for 20 min plus 1.5mg/l mercuric chloride for 5 min with few drops of Tween-20, also. Nodal explants were inoculated on various initiation or establishment media with different combinations of IBA and KIN and the neoformed shoots were cultured on proliferation (multiplication) media for the development of multiple shoots, and the elongation media to elongate of the neoformed shoot. The subsequent elongated shoots were rooted, successfully. The best medium for shoot initiation was Woody Plant Medium (WPM) supplemented with 2.0 mg/l KIN and 1.00 IBA. The favourable medium for multiplication was the tested medium augmented with 5.0 mg/l KIN and 1.00 mg/l IBA.. Furthermore, the in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 1.00 mg/l IBA and NAA ,each in turn (rooting stage). The shoots of Magnolia grandiflora multiplication and rooted successfully when they cultured in WP medium supplemented with 1.00mg/l charcoal. The combination of sand: compost (1:3) was used as substratum for the hardening of the in vitro plantlets, as a potting mix, was the best suited mix for the acclimatization of plantlets.

Ipomoea batatas is an ornamental foliage plant and important for landscaping and interior due to its attractive foliage, easiness to grow and tolerance to low relative humidity and low light conditions. It is a member of the Convulvulaceae family. The goal of this research is to establish a method for initiation of I. batatas plants using single nodal cuttings taken from soft cuttings maintained on full strength MS medium, 30g/l sucrose, 3g/l gelrite, and various doses of plant growth regulators such as NAA, BA, and their combinations. In general, the present study found that when MS medium was fortified with NAA and BA at 1.00 and 0.00 mg/l, respectively, best results for the initiation stage were obtained.

This study was carried out in the Tissue Culture Laboratory, Department of Flowers, Ornamental Plants and Landscape Gardens, Faculty of Agriculture (El-Shatby), Alexandria University, Egypt during the period from 2012 to 2015 to establish an efficient and reliable protocol for in vitro propagation and improve of Jatropha curcas. Seeds of Jatropha were effectiveness of mutation induction by different ways. Planted on Murashige and Skoog media (MS), using different combinations Indol acetic acid (IAA) at 0, 1 and 2 mg/l and benzyl adenine (BA) at 0, 1, 2 and 3 mg/l of kintin (Kin) at 2.0 mg/l and for shoot regeneration .While the media used for rooting was amendmented with different (IAA) at 0, 1 and 2 mg/l and indol butyric acid (IBA) at 0, 1, 2 and 3 mg/l. Oil was extracted from seeds and callus formed on MS medium containing combinations of BA at 0.5, 1 and 1.5 mg/l, (NAA) at 1, 2 and 3 mg/l and 2,4-dichlorophenoxyacetic acide (2,4-D) at 0.5 mg/l. Regenerated rooted plants were then treated by colchicines or glufosinate ammonium at 1, 2, 3 and 4 mg/l for each. The best medium for shoot regeneration was MS amended with BA at 2 and 3 mg / l and IAA at 1mg/l , while the best rooting medium was MS amended with IAA at 2mg/l and IBA at1 and 2mg/l. For obtaining the highest percentage of oil content from callus, medium at 0.5 mg/l BA combined with 2 mg/l NAA and 0.5 mg/l 2,4-D gave the best results. For the induction of genetic variation glyphosinate ammonium to be added at 3 mg/l, while colchicines had at all concatenation no effect on cell division of Jatropha curcas plant.

No abstract

was planned and built between 2005 and 2010. It was established exactly in the entrance of Alexandria city on Cairo-Alexandria desert road occupying an area of about 42000m 2. The hospital has a garden of about 18% of the total area. Since the concept of healing garden is not yet mature in Egypt, an evaluation was conducted between 2010 and 2014 to determine whether the garden was matching the international standards of hospital or healing gardens and whether the garden was meeting the goals and benefits of similar healing gardens. Results from site observations and the statistically analyzed questionnaires indicated several facts: the majority of the tested sample believe that the hospital garden is a very important component of the healthcare facilities. With respect to the general features and components of the gardens, the questionnaires proved that there were points of satisfaction; included the general shape and appearance of the gardens, the general shape of plants and green lawns, garden maintenance and cleaning level which gives a nice pleasant welcome shape to the garden as well as creates a healthy atmosphere for patients and other garden user groups. On the other hand, there were serious points of dissatisfaction including the absence of pergolas, umbrellas and trellises which have been reflected as a problem since there is no way to protect patients and visitors from wind, sunrays or extremes in temperature. Most of the visitors were dissatisfied about the limited variety of plant species and colures, the plants of the garden are not enough to expertise the patients five senses, seeing, smelling, tasting, touching or hearing. The garden users complain, also, because there are no enough walkways with standard characteristics with normal slope for moving wheelchairs. The absence of sitting places in the garden as well as the absence of sculpture and other artistic pieces was one of the serious complains of the garden users. Finally, most of the visitors expressed their dissatisfaction concerning the night lighting of the garden which proved to be insufficient which makes a problem especially for old patients, visually impaired individuals as well as those who have undergone leg bones operations. Based on the results obtained, recommendation for changes was developed to promote better use and benefits of the garden. These research findings can be used to guide the future planning, design, building and subsequent evaluation of garden environments in healthcare facilities.