In Vitro Propagation and Ex Vitro Acclimatization of Magnolia (Magnolia grandiflora, Linn) Trees

Abstract

This study was carried out in the tissue culture laboratory, Faculty of Agriculture, Saba basha, Alexandria University, Egypt during the period from 2013 to 2015. An efficient and reliable protocol for in vitro propagation of Magnolia grandiflora, Linn was optimized. However, nodal explants from field grown of magnolia were used during in vitro culture study for induction of multiple shoots. Nodal explants were effectively surface sterilized with 30% Clorox (sodium hypochlorite) as commercial bleaches for 20 min plus 1.5mg/l mercuric chloride for 5 min with few drops of Tween-20, also. Nodal explants were inoculated on various initiation or establishment media with different combinations of IBA and KIN and the neoformed shoots were cultured on proliferation (multiplication) media for the development of multiple shoots, and the elongation media to elongate of the neoformed shoot. The subsequent elongated shoots were rooted, successfully. The best medium for shoot initiation was Woody Plant Medium (WPM) supplemented with 2.0 mg/l KIN and 1.00 IBA. The favourable medium for multiplication was the tested medium augmented with 5.0 mg/l KIN and 1.00 mg/l IBA.. Furthermore, the in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 1.00 mg/l IBA and NAA ,each in turn (rooting stage). The shoots of Magnolia grandiflora multiplication and rooted successfully when they cultured in WP medium supplemented with 1.00mg/l charcoal. The combination of sand: compost (1:3) was used as substratum for the hardening of the in vitro plantlets, as a potting mix, was the best suited mix for the acclimatization of plantlets.

GC/MS‐Based Fingerprinting Reveals Two Chemotypes in the Leaf Essential Oils fromMagnolia grandifloraTrees within The Urban Forestry of a Colombian Andean Plateau

“…[5,26] M. grandiflora is a popular ornamental tree commonly used as part of forestry in different urban environments because of its attractive shape, shiny green leaves, and fragrant flowers. [27,28] It was introduced in Colombia through different governmental programs to replant trees, and nowadays, it can be found throughout the entire extension of Bogotá city. Beyond a matter of urban landscaping, M. grandiflora trees are essential for Bogotá because they help clean the air (by reducing CO 2 pollution), provide shade, mitigate heavy rains, serve as a habitat for birds, regulate temperature and climate, and collect urban suspended particles.…”

“…M. grandiflora is a popular ornamental tree commonly used as part of forestry in different urban environments because of its attractive shape, shiny green leaves, and fragrant flowers [27,28] . It was introduced in Colombia through different governmental programs to replant trees, and nowadays, it can be found throughout the entire extension of Bogotá city.…”