The epicenter of the COVID-19 crisis since March 17, 2020—the New York metropolitan area—is home to some of the largest Latino immigrant communities in the nation. These communities have long faced barriers to health care access, challenges due to immigration status, and financial and labor instability. The COVID-19 pandemic has aggravated these existing issues in a vulnerable, often forgotten, immigrant community. It has been challenging for this population to access public information regarding COVID-19 testing, treatment, and assistance programs because this information has seldom been disseminated in Spanish and even less frequently in Portuguese. While long-term solutions will require time and changes to policy, some short-term measures can mitigate the current situation. The authors share their experience from Newark, New Jersey, where partnerships of public and private community-based organizations (CBOs) have been successful in establishing trust between the health care system and a fearful Latino community. The Ironbound Initiative, a student group at Rutgers New Jersey Medical School in Newark, New Jersey, has partnered with Mantena Global Care, a Brazilian CBO in Newark, to facilitate dissemination of COVID-19–relevant information. Medical student volunteers, removed from their clinical duties, serve as virtual patient navigators, using social media to reach community members with the goals of improving awareness of precautions to take during the pandemic and of increasing access to needed medical care. These students have collaborated with colleagues in other disciplines to provide necessary legal guidance to community members fearful of seeking care because of their immigration status. The authors urge other academic institutions across the country to recruit multidisciplinary teams of medical, health professions, and law students invested in their local communities and to empower students to partner with CBOs, immigrant community leaders, faith-based organizations, hospitals, and local authorities to support these vulnerable communities during this crisis.
ABSTRACT. Polyploid induction has been used for plant breeding to produce bigger and more robust plants than diploid types. The present study aimed to develop a methodology for in vitro induction of polyploidy in cassava. Apical and lateral microcuttings from the BRS Formosa variety were treated with six oryzalin concentrations for 24 and 48 h. The same methodology was used for colchicine with different concentrations. After 45 days of cultivation and an additional 45 days of subculture, the viability of the explants was assessed and plant acclimatization was performed. Ploidy was determined using flow cytometry. Oryzalin dose and exposure negatively affected cassava explant growth and development compared to untreated explants. Furthermore, apical and lateral explants responded differently to the treatments, showing a diversity in antimitotic sensitivity and effect that is tissue-type specific. In contrast, the doses of 1.25 to 6.25 mM colchicine resulted in high mortality of cassava explants. Therefore, the type of antimitotic affects the morphophysiological behavior of cassava plants in vitro, although apical explants have higher viability and regenerative capacity compared to lateral explants. In addition, the lateral explants have lower mixoploid rates compared to apical explants. Of the 310 plants generated by oryzalin treatments, 277 were diploid, 31 were mixoploid, and 2 were tetraploid. Exposure to oryzalin led to low rate of tetraploids and colchicine caused phytotoxic reactions and death of the explants. The tetraploids were multiplied in vitro to evaluate their yield in the field as well as their behavior against abiotic and biotic stress.
A não existência de uma metodologia adaptada para a multiplicação clonal de C. arabica L. em escala comercial tem sido uma grande restrição a utilização dessa técnica como estratégia para viabilizar o uso de cafeeiros híbridos obtidos pelo melhoramento genético. Com o objetivo de aprimorar as técnicas de micropropagação de brotações de cafeeiro, foram instalados dois experimentos utilizando segmentos nodais de plântulas pré-estabelecidas in vitro de C. arabica. No primeiro experimento estudou-se o efeito de concentrações de BAP (6, 9, 12 e 15 mg dm-3) e ANA (0,5 e 1 mg dm-3) sobre duas cultivares (‘Catuaí Vermelho’ e ‘Maragogipe’), no meio MS. No segundo experimento avaliou-se a influência de concentrações de BAP (0, 6, 9, 12 e 15 mg dm-3) e dois meios de cultura (MS e WPM) sobre três cultivares (‘Catuaí Vermelho’, ‘Icatu’ e ‘Rubi’). Após 90 dias, melhores resultados para as cvs. Catuaí Vermelho e Maragogipe foram obtidos com 15 mg dm-3 de BAP. A cultivar Catuaí Vermelho apresentou maior número de brotações quando comparada com a cv. Maragogipe. Na ausência de BAP, o meio WPM é mais indicado para se obter maior número de brotações da cv. Rubi. Melhores resultados para massa da matéria fresca das brotações verificaram-se em meio MS, para as cvs. Catuaí Vermelho e Icatu.
ABSTRACT:This study aimed to evaluate the agronomic performance of cassava genotypes from the in vitro shoot tip culture to eliminate the cassava frogskin disease for several root and aerial part characteristics. Cassava plants from accessions BGM0315, BGM0464 and BGM0841 infected with cassava frogskin disease were grown in a greenhouse after clonal cleaning. Cuttings from the three accessions were subjected to tetracycline concentrations (0, 5, 10 and 15 mg L -1 ) for three minutes, and then maintained in an acclimatized chamber (35 ± 1 °C and 16 hour photoperiod). Shoots were disinfected for excising shoot tips (0.2 mm and 0.4 mm) and inoculated in a culture medium containing the same concentrations of tetracycline used in the cuttings. After 60 days of cultivation, the explants were transferred to medium without antibiotic, 30 days later they were acclimatized for a period of 70 days for subsequent planting in the field. Seven months after planting, agronomical evaluation was held for root and aerial part characteristics. No influence of isolated shoot tip size was noticed on agronomic characteristics, while the addition of tetracycline in the culture medium, specifically at the concentrations of 5 mg L -1 and 15 mg L -1 , was favorable to the development of the root system of plants in the field. The results revealed that the agronomic performance of cassava plants derived from in vitro cultivation are higher for the production of basic propagation material for the following production cycles, as well as root production for commercial use with subsequent generation of income.
A avaliação da medula óssea é um recurso muito utilizado em Medicina Veterinária para o diagnóstico das doenças do sistema hematopoiético. Foi avaliada a composição celular da medula óssea de cadelas saudáveis, para obter um parâmetro de valores de referência para o mielograma. Na presente pesquisa estabeleceu-se os valores de referencia para mielograma de cadelas saudáveis da Região Metropolitana de Curitiba.
A campanha #VidasNegrasImportam em 2021 ganhou as ruas e as redes sociais denunciando o racismo estrutural e institucional. Foi mobilizada em todo o mundo por conta da morte de George Floyd, homem negro, assassinado pela polícia de Minneapolis, Minnesota, nos Estados Unidos. O objetivo deste artigo é analisar, por meio de uma pesquisa exploratória, as produções e a abordagem da pauta racial pela Radioagência Nacional a partir da busca por cinco palavras-chave: Negro, Racismo, George Floyd, Preconceito e Preto. O estudo está delimitado entre 25 de maio de 2020 e 25 de maio de 2021. Constatamos a visibilidade da pauta racial nas narrativas da agência, mas consideramos que outros temas e abordagens sobre a luta e a resistência da população negra continuam invisibilizados na narrativa jornalística diária.
Resumo: Este artigo trata da midiatização do assédio sofrido por jornalistas nas redações de Imperatriz (MA). O objetivo foi compreender o papel das plataformas digitais nas situações de assédio, já que em levantamento exploratório verificou-se que todas tinham vivido esse tipo de violência no cumprimento de suas funções. Foram ouvidas 19 jornalistas. Conclusivamente, a pesquisa mostra que a sensação de distanciamento que a mediação produz encoraja o assédio e até favorece algumas investidas, mas também tem garantido às profissionais formas de se defender por meio das provas materiais que a ferramenta produz.
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