Present study revealed application of TaqMan real time PCR technique for diagnosis of rabies viral RNA from 11/20 antemortem skin samples with a sensitivity of 92.85%. The successful use of TaqMan real time PCR concluded that it was a viable, alternative tool for the antemortem diagnosis of rabies. Results correlated well with those of the post-mortem fluorescent antibody test performed on brain specimens of same animals.
Aim: Early diagnosis of dreadful rabies is of utmost importance to restrict number of contacts and timely administration of post exposure prophylaxis. The present study was conducted to evaluate the sensitivity comparison of Nested RT-PCR with TaqMan real time PCR technique for intravitam diagnosis of rabies in animals from urine samples.
Materials and Methods:Advance molecular approaches Nested RT-PCR and TaqMan real time PCR was employed on 21 urine samples for intravitam diagnosis of rabies. Comparison of both the techniques was done with standard immunofluorescence test (FAT) applied on brain for postmortem confirmation of rabies.Result: Rabies viral RNA was detected in 6/21 (28.57%) and 11/21 (52.38%) urine samples by application of Nested RT-PCR and TaqMan real time PCR respectively. Sensitivity obtained from both the techniques was 62.50% and 78.94% respectively when compared with gold standard immunofluorescence test (FAT).
Conclusion:TaqMan real time PCR can serve as more sensitive and viable approach for the intravitam diagnosis of rabies as compared to Nested RT-PCR for detection of rabies from urine of suspected animals.
Clinical importance:This study may serve as background for future intravitam rabies diagnostics.
Milk sample was obtained from 17 lactating animals suspected to be rabid. An attempt was made by employing advanced molecular approach Nested RT-PCR and TaqMan real time PCR on milk samples with an aim to detect the presence of rabies viral RNA. Confirmatory diagnosis of rabies was made by applying conventional techniques; Immunofluorescence on brain tissue. This study depicts the presence of rabies viral RNA in 2/17 milk sample with a sensitivity of 54.54% by Nested RT-PCR and 4/17 milk samples with a sensitivity of (60%) by TaqMan real time PCR when compared with FAT applied on brain tissue. This study confirms the presence of rabies viral RNA in milk.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.